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| 反相高效液相色谱法测定生地茶止咳合剂中岩白菜素的含量 | |
| 引用本文： | 付成效,刘高峰,邹渭洪,龙宇,刘琪. 反相高效液相色谱法测定生地茶止咳合剂中岩白菜素的含量[J]. 中南药学, 2012, 10(2): 96-98 |
| 作者姓名： | 付成效 刘高峰 邹渭洪 龙宇 刘琪 |
| 作者单位： | 1. 南华大学附属第一医院药剂科,湖南衡阳,421001 2. 中美上海施贵宝制药有限公司,上海,200240 3. 南华大学附属第二医院药剂科,湖南衡阳,421001 4. 衡阳市中医院药剂科,湖南衡阳,421001 |
| 摘 要： | 目的 建立测定生地茶止咳合剂中岩白菜素含量的反相高效液相色谱法.方法 采用Phenomenex C18-ODS(250 mmm×4.6 mm,5μm)色谱柱,流动相:甲醇-水(23∶77),流速:1.0 mL·min-1,检测波长:275 nm,柱温:30℃.结果 岩白菜素在0.040 8～0.408 μg线性关系良好,(r=0.999 9)；平均回收率为98.5％(n=9).结论 该方法操作简便、快速,适用于生地茶止咳合剂中岩白菜素的含量测定. |
| 关 键 词： | 生地茶止咳合剂 反相高效液相色谱法 岩白菜素 含量测定 |
RP-HPLC determination of bergenin in Shengdicha Zhike Heji |
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| FU Cheng-xiao , LIU Gao-feng , ZOU Wei-hong , LONG Yu , LIU Qi. RP-HPLC determination of bergenin in Shengdicha Zhike Heji[J]. Central South Pharmacy, 2012, 10(2): 96-98 | |
| Authors: | FU Cheng-xiao LIU Gao-feng ZOU Wei-hong LONG Yu LIU Qi |
| Affiliation: | 1.Department of Pharmacy,First Hospital Attached to Nanhua Medical University,Hengyang Hunan 421001;2.Sino-American Shanghai Squibb Pharmaceuticals Ltd,Shanghai 200240;3.Department of Pharmacy,Second Hospital Attached to Nanhua Medical University,Hengyang Hunan 421001;4.Department of Pharmacy,Hengyang Hospital of Traditional Chinese Medicine,Hengyang Hunan 421001) |
| Abstract: | Objective To establish an RP-HPLC method for the determination of bergenin in Shengdicha Zhike Heji.Methods The column was Phenomenex C18-ODS(250 mm×4.6 mm,5 μm).The mobile phase was methanol-water(23∶77) with a flow rate of 1.0 mL·min-1.The detection wavelength was 275 nm.The column temperature was 30 ℃.Results The calibration curve was linear over 0.040 8-0.408 μg(r=0.999 9).The average recovery of bergenin was 98.5%(n=9).Conclusion The method is simple,quick,and suitable for the content determination of bergenin in Shengdicha Zhike Heji. |
| Keywords: | Shengdicha Zhike Heji RP-HPLC bergenin content determination |
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