| 比色法测定酚磺乙胺注射液中抗氧剂的含量 |
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| 引用本文： | 兰文,朱迎军,王伟姣. 比色法测定酚磺乙胺注射液中抗氧剂的含量[J]. 中南药学, 2012, 10(5): 328-331 |
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| 作者姓名： | 兰文 朱迎军 王伟姣 |
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| 作者单位： | 湖南省食品药品检验研究院,长沙,410001 |
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| 基金项目： | 国家药品评价抽验计划质量评价分析项目(编号:2011185) |
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| 摘 要： | 目的 建立分光光度法测定酚磺乙胺注射液中抗氧剂的含量.方法 在EDTA2 -存在的酸性条件下,利用亚硫酸氢根与甲醛和碱性品红作用生成的紫红色复合物,在553 nm处测定吸光度,以此测定亚硫酸氢钠含量.结果 亚硫酸氢钠在8.645～51.873 μg·mL-1吸光度与浓度呈良好的线性关系(r2=0.999 7),平均回收率为97.5％(n=6),RSD为1.4％.结论 本法简便、快速、准确,专属性好,可用于测定酚磺乙胺注射液中抗氧剂的含量.
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| 关 键 词： | 酚磺乙胺注射液 抗氧剂 比色法 含量测定 |
Content determination of antioxidant in etamsylate injection by colorimetric method |
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| LAN Wen , ZHU Ying-jun , WANG Wei-jiao. Content determination of antioxidant in etamsylate injection by colorimetric method[J]. Central South Pharmacy, 2012, 10(5): 328-331 |
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| Authors: | LAN Wen ZHU Ying-jun WANG Wei-jiao |
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| Affiliation: | (Hunan Institute for Food and Drug Control,Changsha 410001) |
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| Abstract: | Objective To establish a colorimetric method to determine the antioxidant content of etamsylate injection.Methods The content of sodium bisulfite was determined by measuring the absorbance of a burgundy complex compound at 553 nm,which was the product reacted by sodium bisulfite,formaldehyde and fuchsin basic in the acidic environment with EDTA.Results The linear range of sodium bisulfite was 8.645-51.873 μg·mL-1(r2=0.999 7).The average recovery was 97.5%(n=6),RSD=1.4%.Conclusion The method is simple,rapid,and accurate,and can be used to determine the antioxidant content of etamsylate injection. |
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| Keywords: | etamsylate injection antioxidant colorimetric method content determination |
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