| 萹蓄高效液相色谱指纹图谱研究 |
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| 引用本文： | 贺凡珍,童欣,刘孟华,苏薇薇.萹蓄高效液相色谱指纹图谱研究[J].中南药学,2012,10(10):773-775. |
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| 作者姓名： | 贺凡珍 童欣 刘孟华 苏薇薇 |
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| 作者单位： | 中山大学生命科学学院,广州,510275 |
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| 基金项目： | 广东省科技计划资助项目(编号:2010B050100013) |
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| 摘 要： | 目的 采用HPLC法建立萹蓄药材的指纹图谱.方法 采用CAPCELL PAK C18 MG(250 mm×4.6 mm,5 μm)色谱柱,以乙腈(A)-0.1％磷酸溶液(B)为流动相,线性梯度洗脱；检测波长340 nm;柱温30℃；流速1.0 mL·min-1.结果 构建的指纹图谱有18个共有峰,确认了其中8个峰的成分分别为绿原酸、杨梅苷、金丝桃苷、木犀草苷、扁蓄苷、槲皮苷、胡桃宁、木犀草素.结论 本研究为萹蓄药材的质量控制提供了新方法.
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| 关 键 词： | 萹蓄 高效液相色谱法 指纹图谱 |
HPLC f ingerprint of Polygonum aviculare L. |
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| HE Fan-zhen
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TONG Xin
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LIU Meng-hua
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SU Wei-wei.HPLC f ingerprint of Polygonum aviculare L.[J].Central South Pharmacy,2012,10(10):773-775. |
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| Authors: | HE Fan-zhen TONG Xin LIU Meng-hua SU Wei-wei |
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| Institution: | (School of Life Sciences,Sun Yat-sen University,Guangzhou 510275) |
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| Abstract: | Objective To establish an HPLC f ingerprint of Polygonum aviculare L..Methods HPLC fingerprint was established with CAPCELL PAK C18 MG(250 mm×4.6 mm,5 μm) column.Acetonitrile(A) and 0.1% phosphoric acid(B) were used as the mobile phase at 1.0 mL · min-1.The detection wavelength and column temperature were 340 nm and 30 ℃respectively.Results In this fingerprint,18 characteristic peaks were selected as the fingerprint peaks.Eight of these characteristic peaks were identified as chlorogenic acid,myricitrin,hyperoside,luteoloside,avicularin,quercitrin,juglanin,luteolin.Conclusion The method can be used for the quality control of Polygonum aviculare L.. |
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| Keywords: | Polygonum aviculare L HPLC fingerprint |
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