甘肃武威地区上胃肠道疾病患者幽门螺杆菌感染与p21ras表达的关系

目的探讨甘肃武威地区上胃肠道疾病患者幽门螺杆菌（Hp）感染与p21ras表达的关系，分析Zip可能的致炎致癌机制。方法Warthin—Starry银染色和革兰染色判断标本中Hp的感染状况；免疫组化S—P法检测标本中p21ras蛋白的表达状况。结果患者中Hp的感染率为83．96％（89／106），胃癌组、胃癌前病变组和浅表性胃炎组的感染率分别为83．33％、92．11％和73．08％，胃癌前病变组Hp感染率显著高于慢性浅表性胃炎组（P〈0．05）。胃癌组、胃癌前病变组和浅表性胃炎组p21ras阳性率分别为87．8％、86．84％和65．38％。胃癌组和癌前病变组p21ras阳性率显著高于浅表性胃炎组（P〈0．05）。Hp阳性组与阴性组之间p21ras阳性率无显著性差别。结论gp感染在武威地区上胃肠道疾病发生和发展中扮有重要角色，胃黏膜病变组织中p21ras的表达与Hp的感染之间无相关性；p21ras的表达在胃癌的发生中发挥作用。

Relationship between Helicobacter pylori infection and the p21ras expression among people suffering from upper gastrointestinal disease in Wuwei, Gansu province

Objective To study the status of Helicobacter pylor （Hp） infection and the relation- ship with the p21ras expression among people suffering from upper gastrointestinal disease in Wuwei, Gansu province in order to analyze the mechanism of the pathogenicity of Hp. Meth- ods The status of Hp infection was detected by Warthin-Starry sliver and Gram stain. The S-P immunohistochemical method was used to detect the p21ras protein expression of the samples. Results The infected rate of Hp among the sufferers was 83.96% （89/106） and in gastric carcinoma group, gastric precancerous lesions group and chronic superficial gastritis group the rates were 83.33%, 92.11% and 73.08% respectively. There existed a significant difference of Hp infection between gastric precancerous lesions group and chronic superficial gastritis group （P 〈0.05）. The p21ras protein positive rates in gastric carcinoma group, gastric precancerous lesions group and chronic superficial gastritis group were 87.8%, 86.84% and 65.38% respectively. The p21ras protein positive rate was significantly higher in gastric carcinoma group and gastric precancerous lesions group than that in chronic superficial gastritis group （P 〈0.05）. There was no significant difference of p21ras protein positive rate between Hp positive group and negative group. Conclusion Hp infection plays an important role in the occurrence and development of upper gastrointestinal disease, p21ras proto-oncogene expression plays an important role in gastric carcinoma occurrence. The carcinogenic mechanism of Hp infection in the area has no relationship with p21ras proto-oncogene expression.