| 黄芪超滤膜提取物对人SGC7901胃癌细胞增殖及转移的影响 |
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| 引用本文： | 付伟,孙志广,蒋立峰.黄芪超滤膜提取物对人SGC7901胃癌细胞增殖及转移的影响[J].山东中医学院学报,2011(3):267-269. |
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| 作者姓名： | 付伟 孙志广 蒋立峰 |
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| 作者单位： | [1]南京中医药大学第一临床医学院,江苏南京210046 [2]甘肃中医学院临床医学系,甘肃兰州710000 [3]江苏省中医院消化科,江苏南京210029 |
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| 基金项目： | 甘肃省中医管理局资助项目（编号：07-GZK-31） |
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| 摘 要： | 目的：研究黄芪超滤膜提取物对人SGC7901胃癌细胞增殖和转移的影响。方法：应用四氮唑蓝比色法（MTT法）测定黄芪超滤膜提取物对人SGC7901胃癌细胞增殖的抑制作用,应用半定量逆转录-聚合酶链反应（RT-PCR）法、Western-Blot法研究黄芪超滤膜提取物对SGC7901胃癌细胞环氧化酶（COX-2）、血管内皮生长因子-c（VEGF-c）表达的影响。结果：黄芪超滤膜提取物对SGC7901胃癌细胞的增殖有一定的抑制作用,使SGC7901胃癌细胞阻滞于G0/G1期,对SGC7901胃癌细胞COX-2、VEGF-c的表达有显著抑制作用。结论：黄芪超滤膜提取物能有效抑制SGC7901胃癌细胞的增殖周期,并可抑制COX-2、VEGF的表达。
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| 关 键 词： | 黄芪超滤膜提取物 胃癌 环氧化酶-2 血管内皮生长因子-c |
Effects of the ultra-filtration extract from astragalus membranaceous on proliferation and metastasis of SGC7901 cells |
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| Authors: | FU Wei SUN Zhi-guang JIANG Li-feng |
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| Institution: | 1.The First Clinical Medical College,Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China;2.The Clinical Medical Department of Gansu College of Traditional Chinese Medicine,Lanzhou 730000,China;3.Gastroenterology Department of Traditional Chinese Medical Hospital of Jiangsu Province,Nanjing 210029,China) |
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| Abstract: | Objective：To study the effects of the ultra-filtration extract from astragalus membranaceous（UEAM） on proliferation and metastasis of SGC7901 cells.Methods：SGC7901 cells proliferation were detected by Tetrazolium assay（MTT） colorimetry.Cell cycle was analyzed by cytometry.Semiquantity reverse translation-polymerase chain reac-tion（RT-PCR） and Western blot were performed to detect the expression of cyclooxygenase-2（COX-2） and vascular endothelial growth factor-c（VEGF-c） in SGC7901 cells.Results：UEAM can inhibit proliferation of SGC7901 cells and arrest SGC7901 cells in G0/G1 period.There were obvious effects on inhibiting expression of COX-2 mRNA and VEGF-c mRNA of SGC7901 cells.Conclusion：UEAM can inhibit proliferation cell cycle,expression of COX-2 mRNA and VEGF-c mRNA of SGC7901 cells. |
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| Keywords: | the ultra-filtration extract from astragalus membranaceous gastric cancer cyclooxygenase-2 vascular endothelial growth factor-c |
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