富含生物碱的7种中草药提取液抑菌活性观察

目的：研究富含生物碱的7种中草药的抑菌活性方法。95％乙醇提取其有效成分后用滤纸片扩散法检测其抑菌活性．等倍稀释法检测其最小抑菌浓度(MIC)．滤纸条法观察协同作用，并测定各提取液生物碱含量。结果：7种中草药均有不同程度的抑菌作用，尤以益母草和苦参效果更强、更普遍；马兜铃与黄芪、马兜铃与益母草、益母草与黄芪对抑制金黄色葡萄球菌，马兜铃与苦参对抑制枯草杆菌分别有协同作用。苦参、山豆根及益母草提取液中生物碱浓度较高。结论：富含生物碱的7种中草药有抑菌活性，作为抗菌资源极具应用前景。

Study on antimicrobial activities of the extract of seven kinds of Chinese Herbs rich in alkaloid

Aim: To study the antimicrobial activities of seven Chinese Herbs rich in alkaloid. Methods: Seven Chinese Herbs rich in alkaloids were extracted with 95% ethanol, and their antimicrobial activities, synergy were studied by paper dispersion or plate methods. Results: All herbs had inhibitory effects, but leonurus heterophyllus sweet and sophora flavescens Ait were the best. There were synergetic effects on staphylococcus aureus among Aristolopchia debilis Sieb. Et zucc and A.mongholicus Bunge, Aristolopchia debilis Sieb. Et zucc and lenourus heteophyllus sweet, leonurus heterophyllus sweet and A.mongholicus Bunge, and on bacillus subtilis between Aristolochia debilis Sieb Et zucc and Sophora flavescens Ait. Conclusion: Seven Chinese Herbs rich in alkaloid have significant antimicrobial activities.