microRNA-34a与乳腺癌的临床病理相关性研究

目的 检测microRNA-34a（miR-34a）在乳腺癌与相应正常组织中的表达情况，研究miR-34a与乳腺癌的临床病理相关性。 方法 应用qRT-PCR技术检测67例乳腺癌标本中miR-34a的表达，同时分析其和临床病理因素之间的相关性。 结果 乳腺癌组织中miR-34a的表达量明显低于配对的癌旁组织（P < 0.01）；年龄、性别、月经状况、孕激素受体水平、Ki67表达与人类表皮生长因子受体水平对病人癌组织miR-34a的表达影响均无统计学意义（P>0.05）；肿块直径、淋巴结转移、病理类型、临床分期、雌激素受体水平、是否三阴性对对病人癌组织miR-34a的表达影响均有统计学意义（P < 0.05~P < 0.01），肿块直径≤2 cm组的表达高于直径>2 cm组（P < 0.05），淋巴结无转移组的表达高于有转移组（P < 0.05），非浸润性组的表达高于浸润性（P < 0.05），Ⅰ期组和Ⅱ期组的表达均高于Ⅲ+Ⅳ期组（P < 0.01），雌激素受体阳性组的表达高于阴性（P < 0.01），三阴性组的表达低于非三阴性组（P < 0.01）。 结论 乳腺癌中miR-34a呈现低表达，与各临床病理因素分析后表明其可能在乳腺癌的发生发展及预后中起重要作用。

Study on the correlation between microRNA-34a level and clinicopathology of breast cancer

Objective To detect the expression levels of microRNA-34a(miR-34a) in breast cancer and corresponding normal tissues, and study the correlation between miR-34a and clinicopathology of breast cancer. Methods The expression levels of miR-34a in 67 breast cancer specimens were detected using qRT-PCR technology, and analyze the correlation between it and clinicopathological factors. Results The expression level of miR-34a in breast cancer tissue was significantly lower than that in matched adjacent tissue(P < 0.01).The differences of the expression levels of miR-34a in cancer tissues among the patients with different ages, sex, menstrual status, progesterone receptor levels, Ki67 expression and human epidermal growth factor receptor levels were not statistically significant(P>0.05).The differences of the expression levels of miR-34a in cancer tissues tumor among the patients with different tumor diametec lymph node metastasis, pathological type, clinical stage, estrogen receptor(ER) level and triple negative were statistically significant(P < 0.05 to P < 0.01).The expression level of miR-34a in tumor diameter ≤ 2 cm group was higher than that in tumor diameter >2 cm group(P < 0.05), the expression level of miR-34a in lymph node without metastasis group was higher than that in lymph node with metastasis group(P < 0.05), and the expression level of miR-34a in non-invasive group was higher than that in invasive group(P < 0.05).The expression level of miR-34a in stage Ⅰ and Ⅱ groups were higher than that in Ⅲ+Ⅳ group(P < 0.01).The expression level of miR-34a in positive ER group was higher than that in negative ER group(P < 0.01), and the expression level of miR-34a in triple negative group was lower than that in non-triple negative group(P < 0.01). Conclusions The expression level of miR-34a in breast cancer is low, and the analysis results of various clinical and pathological factors indicates that it may play an important role in the development and prognosis of breast cancer.