胶体金免疫层析法快速检测CRE碳青霉烯酶的效果评价

目的:分析胶体金免疫层析法对碳青霉烯酶耐药肠杆菌科细菌(CRE)产碳青霉烯酶快速检测的有效性.方法:收集蚌埠医学院第一附属医院临床分离的CRE 80株和对碳青霉烯类抗生素敏感肠杆菌科细菌21株,PCR法检测blaKPC、blaNDM、blaIMP、blaVIM、blaOXA-48耐药基因作为金标准,采用胶体金免疫层析法...

Evaluation on effectiveness of colloidal gold immunochromatography for rapid detection of CRE carbapenems

ObjectiveTo analyze the effectiveness of colloidal gold immunochromatography for rapid detection of carbapenemase-resistant Enterobacteriaceae(CRE) carbapenemase.MethodsA total of 80 CRE strains isolated from The First Affiliated Hospital of Bengbu Medical College and 21 strains of Enterobacteriaceae susceptible to carbapenem antibiotics were collected.PCR was used to detect blaKPC, blaNDM, blaIMP, blaVIM and blaOXA-48 genes as gold standard.Colloidal gold immunochromatography was used to detect the carbapenemase produced by CRE, which was conducted by the consistency analysis and efficiency evaluation combined with PCR results.ResultsAmong 80 CRE strains, 79 strains expressing carbapenemase genes were all positive for the detection of carbapenemase by colloidal gold immunochromatography, which includes 61 strains producing KPC, 10 strains producing NDM, 6 strains producing IMP and 2 strains producing VIM enzymes, the colloidal gold test results of 21 sensitive strains were negative.Compared with the PCR results, the sensitivity and specificity of the four enzymes by colloidal gold immunochromatography were both 100%, and the Kappa value was 1.ConclusionsColloidal gold immunochromatography can be applied as a simple, rapid, sensitive and specific diagnostic method for the detection of CRE carbapenemase, which is significant to the rationalization in the clinical use of antibiotics.