let-7a1和let-7c在非小细胞肺癌中的表达及临床意义

目的:探讨人类微小RNA let-7a1和let-7c在肺癌组织中的表达及与临床病理特征之间的关系。方法:收集53例肺组织手术标本及临床资料,其中包括肺癌组织44例(收集其癌组织和癌旁正常组织)和良性肺疾病组织9例。并收集病人吸烟史、肿瘤TNM分期、病理分化程度及有无淋巴结转移等。采用Trizol法提取总RNA,采用荧光定量PCR检测let-7a1和let-7c的表达,分析其在肺癌组织、癌旁正常组织及良性肺疾病组织中的表达差异及与临床特征的关系。结果:肺癌组织中let-7a1和let-7c的相对表达量均低于癌旁正常组织和良性肺疾病组织(P<0.05);癌旁正常组织中let-7a1和let-7c的相对表达量均低于良性肺疾病组织(P<0.05)。let-7a1表达与分化程度、吸烟和TNM分期有关(P<0.05～P<0.01),与淋巴结有无转移无关(P>0.05)。let-7c表达与淋巴结转移、分化程度有关(P<0.01),与吸烟、TNM分期无关(P>0.05)。结论:let-7a1和let-7c基因在肺癌组织中相对表达量降低,并且随临床分期进展、淋...

Expression and clinical significance of let-7 a1 and let-7 c in non-small cell lung cancer

ObjectiveTo investigate the expression of human microRNA let-7a1 and let-7c in lung cancer tissues and its relationship with clinicopathological features.MethodsThe surgical specimens and clinical data of 53 cases of lung tissue were collected, including 44 cases of lung cancer used to collect cancer tissue and paracancerous normal tissue, and 9 cases of benign lung disease tissue.The smoking history, TNM stage, pathological differentiation and lymph node metastasis were collected.The total RNA was extracted by Trizol method, and the expression of let-7a1 and let-7c was detected by fluorescence quantitative PCR.The difference of let-7a1 and let-7c expression in lung cancer tissue, paracancerous normal tissue and benign lung disease tissue, and its relationship with clinical features were analyzed.ResultsThe relative expression of let-7a1 and let-7c in lung cancer tissue was lower than that in paracancerous normal tissue and benign lung disease tissue(P < 0.05), and the relative expression of let-7a1 and let-7c in paracancerous normal tissue was lower than that in benign lung disease tissue(P < 0.05).The expression of let-7a1 was related to differentiating degree, smoking and TNM stage (P < 0.05 to P < 0.01), but was not related to smoking and TNM stage (P>0.05).The expression of let-7c was related to lymph node metastasis and differentiating degree (P < 0.01), but was not related to smoking and TNM stage (P>0.05).ConclusionsThe relative expression of let-7a1 and let-7c genes in lung cancer tissue decreases, and exhibits decreasing trend with the progress of clinical stage and lymph node metastasis, which suggests that let-7a1 and let-7c are closely related to the occurrence, development and metastasis of lung cancer.Let-7a1 and let-7c may become biomarkers for early diagnosis of lung cancer, and become new targets for targeted therapy of lung cancer, to provide experimental basis for early intervention of high-risk groups.