血清维生素4微量荧光测定法的改进及应用

我们对血清维生素A荧光测定法作了微量化的研究。比较和确定了实验条件。取血50μl,用血清20μl可进行分析。回收率为99.0％。用同一血清样品作10次重复性分析,平均值为23.82±0.126μg/dl(范围23.8-21.2μg/dl)。并作了动物实验和人体应用的观察、证明改进法结果可靠,易为受试者所接受,有利于在营养调查的现场使用。

AN IMPROVED MICROFLUORIMETRIC METHOD FOR MEASURING SERUM VITAMIN A

An improved microfluorimetric method for determination of serum vitamin A was presented. In this method, the excitation wavelength 345nm and emission maximum 480nm were selected, and cyclohexane as solvent for extraction of saponified vitamin A in the serum samples to be tested was used. The sensitivity was sufficient for measurement of vitamin A content in 20*l serum taken from normal fasting blood without elimination of interference. The recovery averaged 99.0% (93.0-105.5).When this method was applied to laboratory study or field work, practically, the results showed that the values of vitamin A obtained seemed to correlate with its nutritional status of experimental animals or subjects investigated. The procedure was simple, reproducible and easily acceptable, espicially in the field work.