A novel digital method to assess air space loss associated with acute lung injury in experimental acute pancreatitis |
| |
| Authors: | R. Mukherjee L. Wen X. Zhang P. Bhattacharya W. Huang R. Sutton |
| |
| Affiliation: | 1. Liverpool Pancreatitis Research Group, Institute of Translational Medicine, University of Liverpool, UK;2. Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital, Sichuan University, Chengdu, China |
| |
| Abstract: | Background/ObjectivesRespiratory dysfunction and/or failure from acute lung injury (ALI) are common in acute pancreatitis (AP), but assessment of ALI in experimental AP has lacked standardisation.MethodsA range of experimental AP models induced in C57BL/6 mice with corresponding controls (n?=?6/group). Full double lung or right lung specimens were taken for histopathological assessment and slides analysed by a pre-set pipeline using Aperio Scanner (Leica), ImageJ software and CellProfiler software. Findings were compared to other routinely assessed parameters.ResultsOverall histopathological changes were similar between both lungs. Mean lung field occupancy was significantly different between moderate and severe CER-AP (21.9% v 27.5%, p? ConclusionWe have developed a novel, simple method for assessment of ALI to improve measurement of systemic organ injury in experimental AP and contribute to preclinical drug development. |
| |
| Keywords: | Acute lung injury Acute pancreatitis |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
