| HPLC法测定小儿解表颗粒中金银花及单味金银花绿原酸的含量 |
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| 引用本文： | 徐雅敏.HPLC法测定小儿解表颗粒中金银花及单味金银花绿原酸的含量[J].湖北中医学院学报,2008,10(4):28-30. |
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| 作者姓名： | 徐雅敏 |
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| 作者单位： | 武汉市普爱医院; |
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| 摘 要： | 目的建立高效液相色谱法测定小儿解表颗粒中绿原酸含量的方法。方法采用Hypersil C18（250mm×4．6mm，5um），色谱柱；以乙腈-0．4％磷酸溶液（13：87）为流动相，流速为1．0mL／min，柱温为室温，检测波长为327nm，以外标法测定金银花药材及小儿解表颗粒中绿原酸的含量。结果绿原酸在11．2～134．4ug·mL^-1内呈线性关系。绿原酸在制剂中的平均回收率（n=5）为99．18％（RSD=0．60％）。结论该方法准确、简便、重现性好，可用于小儿解表颗粒的质量控制。
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| 关 键 词： | 小儿解表颗粒 金银花 绿原酸 高效液相色谱法 |
Determination of Chlorgenic Acid in Flos Lonicerae and Xiaoer JiebiaoGranules by HPLC |
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| XU Ya-ming.Determination of Chlorgenic Acid in Flos Lonicerae and Xiaoer JiebiaoGranules by HPLC[J].Journal of Hubei College of Traditional Chinese Medicine,2008,10(4):28-30. |
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| Authors: | XU Ya-ming |
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| Institution: | XU Ya - ming (Wuhan Pu Ai Hospital, Wuhan , Hubei, 430033) |
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| Abstract: | Objective： To establish the method of determination of chlorogenic acid in Flos Lonicerae and Xiaoer Jiebiao granules by HPLC. Methods： An Hypersil C18 was used with a mobile phase consisted of mixture of acetonitrile- 0. 4% phosphoric acid （13 ： 87） at the flow rate of 1.0mL/min in this method. And UV detector wavelength was 327nm. Results： The linear range of chlorogenic acid was 11.2 - 134.4ug·mL^-1（ r = O. 9999）. The average recovery was 99. 18% （ RSD =0. 60% , n = 5 ）. Conclusion： An accurate, simple, rapid qualitative method with good reproducibility to assay chlorogenic acid in Flos Lonicerae raw material and Xiaoer Jiebiao granules is established. |
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| Keywords: | Xiaoer Jiebiao granules Flos Lonicerae Chlorogenic acid HPLC |
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