蛋白激酶C-δ在不同发育阶段小鼠睾丸的表达

目的　观察蛋白激酶C δ (PKC δ)在不同周龄小鼠睾丸中的表达情况 ,探讨此种PKC同工酶在精子发生中的作用。方法　采用出生后不同周龄的小鼠睾丸组织 ,进行SP免疫组织化学染色。结果　PKC δ在生后睾丸组织中从无到有 ,生后第三周小鼠睾丸中微弱表达 ,第五周开始出现明显的表达量的增加 ,直到第 12周。PKC δ阳性细胞存在于曲细精管中心部位。结论　PKC δ蛋白选择性表达于精子细胞中 ,并且呈现出独特的发育阶段性 ,说明在精子形成过程中这种PKC同工酶具有特殊的作用

The Expression of Protein Kinase C-δ in Testes of Mouse at Various Stages of Development

Objective The expression of Protein Kinase C δ(PKC δ)in testes of mouse at various stages of development was observed to study the function of this PKC isoform in the process of spermatogenesis. Method The testes of mouse at various stages of development were sectioned and stained by SP immunohistochemistry. Result The PKC δ expression was seen weakly in the spermatic cells of the testes of 3 week old, and obvious at the 5 week old until 12 week, perhaps even relatively late in spermatic development. The positive PKC δ cells were observed in the center of the convoluted seminiferous tubule of the testes. Conclusion PKC δ was selectively expressed in spermatids with a unique developmental pattern of expression, the present result suggests that PKC δ may play a specific role in the process of spermatogenesis.