Contractile forces generated by striae distensae fibroblasts embedded in collagen lattices |
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Authors: | Céline?Viennet Jacqueline?Bride Vincent?Armbruster Fran?ois?Aubin Anne-Claude?Gabiot Tijani?Gharbi Email author" target="_blank">Philippe?HumbertEmail author |
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Institution: | 1.Engineering and Cutaneous Biology Laboratory, IFR 133,School of Medicine and Pharmacy,Besan?on,France;2.Optical Laboratory,School of Science,Besan?on,France;3.Department of Dermatology, Engineering and Cutaneous Biology Laboratory,St Jacques Hospital,Besan?on,France |
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Abstract: | Striae distensae are characterized by linear, smooth bands of atrophic-appearing skin that are reddish at first and finally
white. They are due to stretching of the skin, as in rapid weight gain, or mechanical stress, as in weight lifting. The pathogenesis
of striae distensae is unknown but probably relates to changes in the fibroblast phenotype. In order to characterize striae
distensae fibroblasts, alpha-smooth muscle actin expression and contractile forces were studied. Five healthy women with early
erythematous striae and five healthy women with older striae were selected. Paired biopsies were taken from the center of
lesional striae and adjacent normal skin. Fibroblasts were obtained by an explant technique and expanded in vitro in Dulbecco’s
modified Eagle‘s medium. Contractile forces generated by fibroblasts in collagen lattices were measured with the Glasbox device
developed in our laboratory. Alpha-smooth muscle actin expression was studied by immunofluorescence labeling of cells and
by flow cytometry. Fibroblasts from early striae distensae were the richest cells in alpha-smooth muscle actin filaments and
generated the highest contractile forces. Their peak contractile force was 26% greater than normal fibroblasts. There was
a 150% higher level of alpha-smooth muscle actin content in fibroblasts from early striae distensae compared with fibroblasts
from normal skin. In contrast, there was no significant difference in force generation between old striae fibroblasts and
normal fibroblasts with cells expressing no alpha-smooth muscle actin. The contractile properties of fibroblasts from striae
distensae varies depending on the stage of the disease. In early striae distensae, fibroblasts acquire a more contractile
phenotype, corresponding to that of myofibroblasts. |
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Keywords: | Actin Contractile force Fibroblast Striae distensae |
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