ACL TOP 血凝仪两种方法相结合检测纤维蛋白原的分析

目的 评价ACL TOP血凝仪上PT衍算法(PT-der法)和Clauss法相结合检测纤维蛋白原(fibrinogen,Fib)的可行性.方法 分别用PT-der法和Clauss法检测质控血浆的Fib,评价其精密度和准确度,检测90份临床标本并作两种方法的相关性分析.结果 PT-der法和Clauss法的精密度(CV＜10％)和准确度都很好,PT-der法所测结果明显高于Clauss法(P＜0.01),但两者密切相关(r=0.92～0.98),经回归转换后两者结果分布接近.结论 ACL TOP血凝仪上PT-der法和Clauss法相结合检测纤维蛋白原能满足临床需求,既经济又不易漏检异常结果.

Analysis of the combined methods of PT-der and Clauss in plasma fibrinogen detection with ACL TOP coagulation analyzer

Objective To assess the feasibility of the combined methods of PT-der and Clauss in plasma fibrinogen detection with ACL TOP coagulation analyzer.Methods Both PT-der and Clauss methods of ACL TOP coagulation analyzer were used to detect the fibrinogen levels in quality control plasma samples.The accuracy and precision of the two methods were compared.Ninety clinical samples were detected to analyze the correlation of the two methods.Results Both methods have good accuracy and precision(CV<10%) in plasma fibrinogen detection.The result of PT-der method was significantly higher than that of Clauss method(P<0.01),but a closely correlation was found between these two methods,and the results of them were found to have a similar distribution by a regression transition.Conclusion With ACL TOP coagulation analyzer,the combined methods of PT-der and Clauss can meet the normal requirements in clinical plasma fibrinogen detection with economical benefit and low detection miss rate.