| Abstract: | Objectire: To study molecular mechanism of suppressive effect of macrophages posttrauma on T cell functions.
Methods: A murine dosed trauma model was used, macrophages were harvested from the abdominal cavity and added into the culture system of T cells, which were separated from splenocytes in normal mice using nylon column. T cell functions and intracellular messenger molecules were determined. In addition, the effect of macrophages' removal from splenocytes of traumatized mice on T cell functions and intracellular messenger molecules was investigated.
Results: Macrophages posttrauma in vitro could obviously suppress ConA stimulated normal T cell functions such as T lymphocyte transformation, interleukfin 2 (IL-2)production, IL-2 receptor α (IL-2Rα) expression, IL-2mRNA and IL-2Rα mRNA levels, and elevate cAMP contents of activated normal T cells while decreasing cGMP contents, intracellular free calcium ( Ca2+ ]i) concentration and protein kinase C (PKC) activity. Removal of macrophages from splenocytes of traumatized mice could at certain degree reverse the suppression of T cell functions, decrease cAMP contents while increasing cGMP contents, Ca2+ ]i concentration and PKC activity.
Conclusions: Macrophages posttrauma may suppress T cell functions via altering messenger molecule levels in activated T cells. |
