用HPLC法测定大鼠血浆和胆汁中芹菜素的浓度

目的：建立HPLC法测定静脉给药后大鼠体内的芹菜素浓度。方法：采用HPLC法，色谱柱为Dikma C18柱，流动相为甲醇：0．2％（V／V）磷酸水溶液-66．5：33．5，检测波长350nm。用该方法测定5只大鼠静脉给药后不同时间点的血浆及胆汁中芹菜素浓度，计算主要药动学参数及胆汁排泄率。结果：血浆药物浓度的标准曲线在0．04～8μg／mL范围内线性关系良好（r=0．9998），胆汁药物浓度标准曲线在0．25～8μg／mL范围内线性关系良好（r=0．9998），血浆和胆汁的精密度和方法回收率均良好。芹菜素静脉给药后符合二室模型，消除半衰期为95．2min，胆汁排泄率为5．64％。结论：该方法灵敏、快速、准确，能够用于芹菜素的药动学研究。芹菜素静脉给药后，血浆浓度迅速下降，部分通过胆汁排泄。

Determination of the concentration of apigenin in rat plasma and bile by HPLC method

Objective： To establish a HPLC method to determine the concentration of apigenin in rat plasma and bile. Methods： Dikma C18 column and methanol-0.2 % （V/V） phosphoric acid water solution（66.5 ： 33.5） as the mobile phase were used. The detection wavelength was 350 nm. The concentration of apigenin in plasma and bile were determined by HPLC method after single dose of 20 mg/kg iv. Pharmacokinetic parameters and bile excretion rate were calculated. Results：The calibration curve of apigenin in rat plasma was linear within the range of 0.04 8 μg/mL（r=0. 999 8） ,and in rat bile was linear within the range of 0.25-8 μg/mL（r= 0.999 8）. The precision and recovery of apigenin both in rat plasma and bile were good. The concentrationtime curve of apigenin conformed to a two-compartment model and the bile excretion rate was 5.64 %. Conclusion：The method is sensitive, rapid,accurate and suitable for the quantitative determination of apigenin in plasma and bile. It is a potential method for pharmacokinetic studies of apigenin. The concentration of apigenin in plasma declined rapidly after iv injection and part of apigenin was excreted from bile.