| 无血清培养体系中CD3AK细胞诱导HL—60细胞凋亡的研究 |
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| 引用本文： | 李光金,陈功星,严维耀,郑兆鑫. 无血清培养体系中CD3AK细胞诱导HL—60细胞凋亡的研究[J]. 中国免疫学杂志, 2001, 17(7): 353-355 |
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| 作者姓名： | 李光金 陈功星 严维耀 郑兆鑫 |
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| 作者单位： | [1]复理大学生命科学学院遗传工程国家重点实验室，上海200433 [2]复理大学生命科学学院遗传工程国家重点实验室，上 |
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| 摘 要： | 目的：采用无血清培养体系，研究CD3单克隆抗体激活的杀伤（CD3AK）细胞诱导HL－60细胞凋亡情况。方法：用血清代用品代替人血清培养CD3AK细胞，将诱导生成的CD3AK细胞与HL－60细胞共同孵育后，通过观察细胞形态、琼脂糖凝胶电泳检测DNA条带及充式细胞仪（FCM）检测分析HL－60细胞凋亡情况。结果：与CD3AK细胞孵育7h后的HL－60细胞出现凋亡细胞形态特征，DNA凝胶电泳典型梯状电泳带，FCM检测凋亡细胞比例为24.59%。结论：无血清培养条件下CD3AK细胞可诱导HL－60细胞凋亡。
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| 关 键 词： | 无血清培养 CD3单克隆抗体 细胞凋亡 效应细胞 粒酶 白血病 HL－60细胞 |
| 文章编号： | 1000-484X(2001)07-0353-03 |
Study on HL-60 cell apoptosis induced by CD3 McAb-activated killer cells in serum-free culture system |
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| LI Guang Jin,CHEN Gong Xing,YAN Wei Yao et al.State Key Laboratory of Genetic Engineering,Fudan University,Shanghai. Study on HL-60 cell apoptosis induced by CD3 McAb-activated killer cells in serum-free culture system[J]. Chinese Journal of Immunology, 2001, 17(7): 353-355 |
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| Authors: | LI Guang Jin CHEN Gong Xing YAN Wei Yao et al.State Key Laboratory of Genetic Engineering Fudan University Shanghai |
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| Abstract: | Objective:To investigate whether CD3McAb activated killer(CD3AK)cells could induce apoptosis in HL 60 cells in serum free culture system.Methods:HL 60 cells were coincubated with CD3AK cells expanded in serum free medium,then apoptosis was assessed by means of morphological studies,DNA agarose gel electrophoresis and flow cytomether(FCM) analysis.Results:After 7 h of coincubation with CD3AK cells in serum free medium,HL 60 cells showed morphological features of apoptosis and typical DNA ladder on gel electrophoresis.FCM analysis showed that 24.59% cells were found to undergo apoptosis.Conclusion:CD3AK cells can induce apoptosis in HL 60 cells under serum free culture conditions. |
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| Keywords: | Serum free culture Anti CD3 monoclonal antibody Apoptosis Granzyme |
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