Radiation-sterilized insoluble collagenous bone matrix is a functional carrier of osteogenin for bone induction |
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| Authors: | Ronald W Katz Greg C Felthousen A Haridara Reddi |
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| Institution: | (1) Department of Graduate Periodontology, University of Maryland College of Dental Surgery, Baltimore;(2) Bone Cell Biology Section, National Institute for Dental Research, National Institutes of Health, Bethesda, Maryland, USA;(3) Clinical Investigations and Patient Care Branch, National Institute of Dental Research, National Institutes of Health, 20014 Bethesda, MD, USA |
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| Abstract: | Summary The influence of gamma radiation on the role of the collagenous substratum as a carrier for proteins which cause bone induction
was examined. Osteoinductive demineralized bone matrix was extracted by 4 M guanidinium hydrochloride. The insoluble collagenous
bone matrix (ICBM) obtained was not osteoinductive; however, when reconstituted with partially purified osteogenin, bone induction
was restored. In order to apply the principle of bone induction to clinical use, methods of sterilization must be optimized
to maintain the osteoinductive activity of bone allografts. The inactive substratum was irradiated and reconstituted with
an active, partially purified bone extract and bioassayed. Irradiation of the ICBM by a Cobalt 60 source at a dose of 1 and
3 Mrads had no deleterious effect on the functional role of the substratum. |
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| Keywords: | Osteogenin Collagenous Substratum Radiation Alkaline Phosphatase |
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