改良精子染色质扩散实验对精子DNA完整性检测的初步探讨

目的:通过对传统精子染色质扩散实验(SCD)方法进行改良,探索一种更简单、快速、准确的精子DNA完整性检测方法。同时对改良SCD法检测的正常参考值范围进行初步探讨。方法:对传统SCD法在实验过程、试剂配制及器具使用上进行了改良优化,同时对改良前后SCD法进行了比较。此外,利用改良SCD法对293例正常生育男性精液进行精子DNA完整性检测。结果:建立了改良SCD检测法,经统计分析改良前后测定结果无统计学差异,同时初步对改良SCD法检测的正常精子DNA百分比参考值范围进行了确定,其值为精子DNA异常发生率<32.58%。结论:改良SCD方法能更快速地对精子DNA的完整性进行检测,同时,由于降低了试剂成本,该法更容易在实际工作中得到普及。

Determination of Sperm DNA Integrity by Improved Sperm Chromatin Structure Assay Test

Objective:To search for a simple,quick and accurate method for the sperm DNA integrity test by modifyiag the conven-tional sperm chromatin dispersion(SCD)test,and to investigate the reference value range of normal sperm DNA integrity by the im-proved SCD assay. Methods:We modified the conventional SCD test in its procedures,reagents preparing and utensils to be used,compared the improved method with the conventional one,and detected 293 normal semen samples for sperm DNA integrity by the modified SCD test. Results:An improved SCD test was established,and no statistical differences were found between the results of the modified and conventional methods.The reference value range was determined by the improved SCD assay for normal sperm DNA integrity,which was<32.58% for abnormal sperm DNA integrity. Conclusion:The improved SCD test could detect sperm DNA integrity more quickly,and it reduced the cost in reagents.