妊娠高血压综合征患者血浆内源性一氧化碳、内皮素-1、全血血小板聚集性检测

目的:检测妊娠高血压综合征(妊高征)患者血浆内源性一氧化碳(CO)、内皮素-1(ET-1)、血小板聚集性(PAg)的水平.方法:分别采用联二亚硫酸盐法、放射免疫法和血小板聚集仪测定20例健康晚孕妇女与61例妊高征患者(轻度19例,中度20例,重度22例)血浆CO、ET-1、全血PAg水平,并进行相关性分析.结果:中、重度妊高征组CO水平较正常晚孕组下降(P＜0.01或0.001),重度组较轻度组下降(P＜0.01);妊高征各组血浆ET-1与全血PAg水平均较正常晚孕组升高(P＜0.05或0.001),重度组较轻度组升高(P＜0.01);血浆CO水平与ET-1、PAg间均呈负相关(P＜0.05或0.001).结论:CO可能通过拮抗ET-1的生成或释放、抑制血小板聚集而存妊高征的病理生理过程中发挥重要的生理作用.

Detection of plasma endogenous carbon monoxide, endothlin-1, and platelet aggregation in patients with pregnancy induced hypertension

Aim: To study the role of CO in pathophysiological changes of pregnancy induced hypertension (PIH).Methods: Plasma levels of carbon monoxide(CO), endothlin-1(ET-1) and platelet aggregation(PAg) in 20 cases of normal late pregnant women and 61 cases of patients with PIH (19 cases of mild PIH,20 cases of moderate PIH,and 22 cases of severe PIH) were measured by Chalmers dithionite reduction methods,radioimmunoassay, and platelet aggregation machine, respectively.Results:Compared with normal pregnant women,plasma CO level in PIH groups decreased (P<0.01 or 0.001). The plasma ET-1 level and platelet aggregation were higher in PIH groups than those of normal pregnant women(P<0.05 or 0.001).The plasma CO level was negatively correlated with ET-1 and platelet aggregation in all groups(P<0.05 or 0.001).Conclusion: CO may be one of the antagonists of ET-1 and platelet aggregation in the pathogenesis of PIH.