循环miR-135a水平与HDL抗氧化功能的关联性

目的 研究血浆高密度脂蛋白(HDL)功能与循环微小RNA(miRNA)水平的相关性.方法 利用失功能HDL模型小鼠清道夫受体B族Ⅰ型缺陷(SR-B Ⅰ-/-)小鼠发掘特异性miRNA谱的变化;收集30例2020年9月-2021年5月就诊于武汉大学中南医院心血管内科的冠状动脉粥样硬化性心脏病(CAD)患者血浆并检测miR...

Correlation between circulating miR-135a level and antioxidant function of HDL

Aim To study the correlation between the antioxidantion of plasma high density lipoprotein(HDL)and circulating microRNA(miRNA).Methods Scavenger receptor group B typeⅠdeficient(SR-BⅠ^-/-)mice as dysfunctional HDL model mice were used to explore the changes of specific miRNA spectrum.From September 2020 to May 2021,30 patients with coronary artery disease(CAD)were enrolled in the Department of Cardiology,Zhongnan Hospital of Wuhan University,non-CAD patients in the same period as control group,both groups with HDL cholesterol(HDLC)higher than 1.04 mmol/L.Results Compared with wild-type mice,the plasma miR-135a in SR-BⅠ^-/-mice decreased significantly.Plasma paraoxonase 1(PON-1)activity decreased in CAD patients,PON-1 activity in control group was positively correlated with HDLC level,but not in CAD group.The plasma miR-135a level in CAD patients also decreased,and was positively correlated with PON-1 activity.In CAD group,the level of plasma miR-17 decreased significantly,and the levels of miR-223 and miR-760 increased significantly,but there was no correlation with HDLC level or plasma PON-1 activity.Conclusion The level of plasma miR-135a decreased significantly in CAD patients with high HDLC,and was positively correlated with PON-1 activity,which may be related to the antioxidant function of HDL.