跑台运动对脑卒中后抑郁大鼠抑郁样行为、海马氧化应激及海马CA1区BDNF表达的影响

目的 观察脑卒中慢性应激大鼠抑郁样行为、海马氧化应激及海马CA1区脑源性神经营养因子(BDNF)表达的变化和中等强度跑台运动的干预作用,探讨跑台运动影响卒中后抑郁(PSD)大鼠抑郁行为的可能机制.方法 将44只大鼠随机分为4组:即假手术组(sham)、卒中组(MCAO)、卒中抑郁组(PSD)及PSD运动组(PSD+E)...

Effects of treadmill exercise on depressive behavior,and hippocampal oxidative stress and CA1 BDNF expression,in post-stroke depression rats

Objective To observe changes in depressive-like behavior,and expression of oxidative stress markers and brain-derived neurotrophic factor(BDNF)in the CA1 hippocampus in post-stroke depression(PSD)model rats,and to investigate intervention with treadmill exercise in these rats and its possible mechanisms.Methods Forty-four male Sprague-Dawley rats were randomly divided into the following four groups:sham-operation,middle cerebral artery occlusion(MCAO),PSD,and PSD rats treated with 4 weeks of treadmill exercise only(PSD+E).Complete cerebral ischemic rat models of PSD were established by MCAO followed by 4 weeks of chronic unpredictable mild stimulation.Changes in rat behavior were evaluated by forced swimming test(FST)and sugar water preference test(SPT).The activities of superoxide dismutase(SOD)and catalase(CAT),and the levels of malondialdehyde(MDA)in the hippocampus,were detected using kits.Immunohistochemical staining was used to detect BDNF expression in the CA1 hippocampus.Results Compared with the sham group,there was prolongation of the FST immobility time,while sucrose water intake and sucrose consumption ratio in the SPT were significantly decreased in the group.In the PSD group,there was reduced SOD and CAT activity,and increased MDA concentration,in the hippocampus,while the level of BDNF in the hippocampal CA1 of rats was significantly reduced compared with the group.Compared with the PSD group,the PSD+E rats had shorter FST immobility times,significantly higher sucrose water intake and sucrose consumption ratios in the SPT,enhanced SOD and CAT activity,decreased MDA concentration in the hippocampus,and significantly increased expression of BDNF in the hippocampal CA1.Conclusions Treadmill exercise alleviated depressive-like behavior and conferred neuroprotection by reinforcing antioxidant capability in the hippocampus and activating BDNF expression in the hippocampal CA1 of PSD model rats.