高脂喂养对载脂蛋白O基因敲除小鼠表型的影响

目的 探究载脂蛋白O(ApoO)基因敲除促进高脂饮食诱导的肥胖及代谢紊乱表现.方法 将ApoO基因敲除杂合小鼠进行配种繁殖,提取子代小鼠的组织DNA,用PCR技术检测小鼠的基因型.实时荧光定量PCR和Western blot检测ApoO基因敲除mRNA和蛋白表达水平.饲喂高脂饮食后,通过小鼠体型、摄食量、肝脏脂质组学等...

Effect of high fat feeding on phenotype of apolipoprotein O in knockout mice

Aim To investigate the effect of apolipoprotein O knockout mice promoting obesity and metabolic disorders challenged by high fat diet(HFD).Methods Breeding the ApoO heterozygous mice will get the homozygous mice offspring.PCR reaction was used to detect the gene genotype after extracting the mice tissue DNA.mRNA and protein expression of ApoO were determined by quantitative real-time PCR(qRT-PCR)and Western blot assay,respectively.After feeding with high-fat diet,the metabolic phenotypes in mice were observed by somatotype,food intake,lipidomic study in liver.Results The homozygous mice offspring were obtained.It was found that homozygous mice had obesity and more severe hepatic steatosis after HFD challenging.Lipidomics of the liver revealed obvious accumulation of triglyceride and significant changes of phospholipid components.Conclusion The deletion of ApoO may promote obesity and metabolic disorders induced by high-fat diet,which could provide a new target for the prevention and treatment of metabolic syndrome and cardiovascular diseases.