| DOX调控HNRNPK稳定下调的H1299细胞株的构建与研究 |
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| 引用本文： | 李梦媛,杨星九,张文龙,李维莎,曹琳,刘宏飞,高苒. DOX调控HNRNPK稳定下调的H1299细胞株的构建与研究[J]. 中国比较医学杂志, 2021, 0(1) |
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| 作者姓名： | 李梦媛 杨星九 张文龙 李维莎 曹琳 刘宏飞 高苒 |
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| 作者单位： | 中国医学科学院医学实验动物研究所 |
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| 基金项目： | 中国医学科学院医学与健康科技创新工程-重大协同创新项目-协同创新团队资助(2016-I2M-3-019)。 |
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| 摘 要： | 目的构建DOX调控HNRNPK稳定下调的H1299细胞株,并研究HNRNPK下调对细胞增殖与凋亡的影响。方法用包装好的慢病毒感染H1299细胞,感染72 h后利用嘌呤霉素筛选阳性细胞并扩大培养获得稳转株,通过定量PCR与Western blot检测HNRNPK表达效率,并研究HNRNPK下调对细胞增殖与凋亡的影响。结果成功构建了DOX调控HNRNPK稳定下调的H1299细胞株,HNRNPK的下调抑制了H1299细胞的增殖,但对其凋亡没有明显影响。结论 HNRNPK下调抑制了H1299细胞的增殖。
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| 关 键 词： | HNRNPK DOX 细胞增殖 细胞凋亡 |
Establishment and analysis of the H1299 cell line for HNRNPK downregulated by DOX |
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| LI Mengyuan,YANG Xingjiu,ZHANG Wenlong,LI Weisha,CAO Lin,LIU Hongfei,GAO Ran. Establishment and analysis of the H1299 cell line for HNRNPK downregulated by DOX[J]. Chinese Journal of Comparative Medicine, 2021, 0(1) |
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| Authors: | LI Mengyuan YANG Xingjiu ZHANG Wenlong LI Weisha CAO Lin LIU Hongfei GAO Ran |
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| Affiliation: | (Institute of Laboratory Animal Science,Chinese Academy of Medical Science&Comparative Medical Center,Peking Union Medical College,NHC Key Laboratory of Human Disease Comparative Medicine Beijing,Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases,Bejing 100021,China) |
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| Abstract: | Objective To establish the H1299 cell line with HNRNPK downregulated by DOX and study the effect of HNRNPK downregulation on cell proliferation and apoptosis.Methods H1299 cells were infected with a packaged lentivirus.After 72 h,positive cells were selected by puromycin and expanded to a stable transgenic cell line.The expression efficiency of HNRNPK was detected by q PCR and Western blot.The effects of HNRNPK downregulation on cell proliferation and apoptosis were analyzed.Results The H1299 cell line with HNRNPK downregulated by DOX was established successfully.Downregulation of HNRNPK inhibited the proliferation of H1299 cells but did not affect apoptosis.Conclusions Downregulation of HNRNPK inhibits the proliferation of H1299 cells. |
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| Keywords: | HNRNPK DOX cell proliferation apoptosis |
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