| p38 MAPK对PDGF诱导的细胞迁移的调控作用 |
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| 引用本文： | 龚小卫,魏洁,李煜生,程蔚蔚,邓鹏,姜勇. p38 MAPK对PDGF诱导的细胞迁移的调控作用[J]. 解放军医学杂志, 2007, 32(3): 205-207 |
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| 作者姓名： | 龚小卫 魏洁 李煜生 程蔚蔚 邓鹏 姜勇 |
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| 作者单位： | 510515,广州,南方医科大学病理生理学教研室和广东省蛋白质组学重点实验室;510515,广州,南方医科大学病理生理学教研室和广东省蛋白质组学重点实验室;510515,广州,南方医科大学病理生理学教研室和广东省蛋白质组学重点实验室;510515,广州,南方医科大学病理生理学教研室和广东省蛋白质组学重点实验室;510515,广州,南方医科大学病理生理学教研室和广东省蛋白质组学重点实验室;510515,广州,南方医科大学病理生理学教研室和广东省蛋白质组学重点实验室 |
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| 基金项目： | 国家自然科学基金 , 广东省科技厅科技计划 , 广东省医学科学技术研究基金 |
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| 摘 要： | 目的 研究p38丝裂原活化蛋白激酶(MAPK)在血小板源性生长因子(PDGF)诱导的细胞迁移中的作用.方法 PDGF刺激小鼠NIH 3T3细胞后,利用免疫印迹法检测p38磷酸化程度的改变情况.利用Transwell细胞迁移系统来观察PDGF对NIH 3T3细胞迁移的诱导作用,以及p38基因敲除对PDGF诱导的细胞迁移的影响.结果 PDGF能显著诱导NIH 3T3细胞迁移(P＜0.001),且在此过程中p38能被磷酸化激活,p38基因敲除能阻断PDGF诱导的细胞迁移(P＜0.001).结论 p38 MAPK参与了PDGF诱导的细胞迁移的调控.
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| 关 键 词： | p38 MAP激酶 血小板源生长因子 细胞运动 |
| 收稿时间： | 2006-12-22 |
| 修稿时间： | 2007-02-08 |
p38 MAPK is involved in the regulation of PDGF-induced cell migration |
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| Gong Xiaowei, Wei Jie, Li Yusheng,et al.. p38 MAPK is involved in the regulation of PDGF-induced cell migration[J]. Medical Journal of Chinese People's Liberation Army, 2007, 32(3): 205-207 |
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| Authors: | Gong Xiaowei Wei Jie Li Yusheng et al. |
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| Affiliation: | Gong Xiaowei, Wei Jie, Li Yusheng, et al. |
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| Abstract: | Objective To investigate the role of p38 mitogen-activated protein kinase(MAPK)in the cell migration induced by platelet-derived growth factor(PDGF).Methods The phosphorylation degree of p38 in NIH 3T3 cell line,which was stimulated with PDGF,was analyzed with Western blot.The effects of PDGF treatment on the induction of the migration of NIH 3T3 cells,as well as p38 gene knockout on the PDGF-induced cell migration,were observed by using Transwell cell migration system.Results The migration of NIH 3T3 cells was significantly induced by PDGF treatment(P<0.001),and in this process,p38 was activated by phosphorylation.Furthermore,the cell migration induced by PDGF was inhibited by p38 gene knockout(P<0.001),as showed in the experiment performed with p38 gene knockout mouse embryonic fibroblasts.Conclusion p38 MAPK plays an important role in the regulation of cell migration induced by PDGF. |
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| Keywords: | p38 MAP kinase platelet-derived growth factor cell movement |
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