| 高效液相色谱法测定兔血清中丁螺环酮的浓度 |
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| 引用本文： | 赵维娟,边佳明,许景峰. 高效液相色谱法测定兔血清中丁螺环酮的浓度[J]. 中国药房, 2003, 14(10): 590-591 |
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| 作者姓名： | 赵维娟 边佳明 许景峰 |
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| 作者单位： | 北京军区总医院药理科,北京市,100700 |
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| 摘 要： | 目的 :建立高效液相色谱法测定兔血清中丁螺环酮浓度的方法。方法 :采用ZORBAXSB -C18 色谱柱 ,以甲醇 -乙腈 -水 -0 1mol/L醋酸钠缓冲液 (pH4 5) (10∶38∶51∶1)为流动相 ,检测波长为237nm。以峰面积内标法测定浓度。结果 :本方法能有效分离被测组分 ,其丁螺环酮在1～80ng/ml浓度范围内与峰面积呈现良好的线性关系 ,r=0 9979。结论 :本方法操作简便 ,测定结果准确 ,灵敏度高 ,适用于血清药物浓度测定
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| 关 键 词： | 高效液相色谱法 丁螺环酮 血药浓度 |
| 文章编号： | 1001-0408(2003)10-0590-02 |
| 修稿时间： | 2002-06-26 |
Determination of the Serum Concentration of Buspirone by HPLC in Rabbits |
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| ZHAO Weijuan,BIAN Jiaming,XU Jingfeng. Determination of the Serum Concentration of Buspirone by HPLC in Rabbits[J]. China Pharmacy, 2003, 14(10): 590-591 |
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| Authors: | ZHAO Weijuan BIAN Jiaming XU Jingfeng |
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| Abstract: | OBJECTIVE:To establish a HPLC method for determination of the concentration of Buspirone in serum.MET_ HODS:The chromatographic system consisted of ZORBA SB-C 18 column and mobile phase of methanol-acetonitrile-water-0.1mol/L NaAC buffer solution(pH4.5)(10∶38∶51∶1),with a detection wavelength of237nm,and the content was calcuˉlated by peak area internal standard method.RESULTS:The linear range of Buspirone was1～80ng/ml,r=0.9979.CONCL_ USION:This method is simple and accurate for determination of Buspirone in serum. |
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| Keywords: | HPLC Buspirone serum drug concentration |
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