| Fas转导大肠癌细胞诱导其凋亡的实验研究 |
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| 引用本文： | 李恕军,韩英,姜泊,肖冰. Fas转导大肠癌细胞诱导其凋亡的实验研究[J]. 中国医药导刊, 2003, 5(2): 90-91 |
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| 作者姓名： | 李恕军 韩英 姜泊 肖冰 |
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| 作者单位： | 1. 北京军区总医院消化内科,北京,100700
2. 第一军医大学南方医院消化内科,广州,510515 |
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| 摘 要： | 目的：观察Fas转导大肠癌细胞株诱导大肠癌细胞凋亡效应，可望为进一步研究大肠癌的Fas基因治疗提供实验依据。方法：以流式细胞术(FCM)及琼脂糖凝胶电泳法捡测Fas转导大肠癌细胞调亡效应。实验分四组：a.LoVo细胞组b．LoVo细胞 Fas抗体组c.LOVo转导细胞组dLoVo转导细胞 Fas抗体组。结果：Fas转导大肠癌细胞株 Fas抗体组(d)细胞凋亡率明显高于a,b,c组。结论：Fas表达细胞株在Fas抗体作用下能够有效启动Fas介导的细胞凋亡途径。
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| 关 键 词： | 大肠癌 癌细胞凋亡 Fas基因 基因转导 流式细胞术 琼脂糖凝胶电泳法 |
The Study of Apoptosis in Colorectal Cancer Cells Induced by Fas Gene Transduction |
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| Li Shu -jun,Han ying,Jiang Bo Xiao Bing. The Study of Apoptosis in Colorectal Cancer Cells Induced by Fas Gene Transduction[J]. Chinese Journal of Medicinal Guide, 2003, 5(2): 90-91 |
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| Authors: | Li Shu -jun Han ying Jiang Bo Xiao Bing |
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| Abstract: | Objective: To observe the apoptosis in colorectal cancer cells induced by Fas gene transduction, hoping to provide evidiences for Fas gene therapy of colorectal cancer. Methods: The apoptosis of Fas - expressed colorectal cancer cells were detected by flow cytornetry and agarose gel electrophoresis.There were 4 groups,a. LoVo cells,b. LoVo cells + Fas antibody c. LoVo Fas cells d. LoVo Fas cells + Fas antibody. Results: The apoptotic rate of LoVo Fas cells + Fas antibody group(d) was much higher than that of those three groups. Conclusion: Apoptosis in Fas - expressed colorectal cancer cells can be induced effectively by Fas gene through the function of Fas antibody. |
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| Keywords: | Fas gene transduction colorectal cancer apoptosis |
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