EPCAM、CD44和CD24在胃癌组织中的表达及其临床意义

目的: 联合检测95例胃癌组织中上皮细胞黏附分子(EPCAM)、白细胞分化抗原 44(CD44)和白细胞分化抗原 24(CD24)的表达情况,分析这3种蛋白与胃癌临床病理资料及预后之间的关系。方法: 应用免疫组织化学法检测95例经手术切除并有明确病理诊断为胃癌的标本中EPCAM、CD44和CD24的表达。分析95例胃癌临床病理资料与这3种蛋白阳性表达之间的关系。结果: (1) EPCAM阳性56例(58.95%),CD44阳性41例(43.16%),CD24阳性56例(58.95%)。其中EPCAM⁺CD44⁺30例(31.58%),EPCAM⁺CD24⁺45例(47.37%),CD44⁺CD24⁺32例(33.68%),EPCAM⁺CD44⁺CD24⁺25例(26.32%)。(2)EPCAM与年龄、肿瘤浸润深度、WHO组织学分型有关;CD44与BORRMANN分型、WHO组织学分型、CEA值有关;CD24与浸润深度、肿瘤位置、WHO组织学分型、脏器侵犯有关;三者阳性与浸润深度、肿瘤位置、WHO组织学分型有关(P<0.05)。(3)EPCAM、CD44阳性组的生存率与阴性组比较差异有统计学意义(P<0.05及P<0.01);EPCAM⁺CD44⁺CD24⁺与EPCAM^-CD44^-CD24^-的生存率比较差异有统计学意义(P<0.05); EPCAM^-CD44⁺CD24⁺与EPCAM^-CD44^-CD24^-的生存率比较有统计学意义(P<0.05)。结论: EPCAM、CD44和CD24在胃癌组织中表达阳性率高,可作为胃癌诊断的初筛实验。

Expression of EPCAM,CD44 and CD24 in gastric cancer tissues and its clinical significance

AIM: To evaluate the relationship between epithelial cell adhesion molecule(EPCAM)/cluster of differentiation 44(CD44)/cluster of differentiation 24(CD24) expression and the clinicopathological characteristics/prognosis in 95 gastric cancer patients.METHODS: The expression levels of EPCAM,CD44 and CD24 were detected using the two-step method of immunohistochemistry in 95 gastric cancer patients who underwent surgical excision and were pathologically diagnosed as gastric cancer.RESULTS: There were 56 EPCAM-positive patients(58.95%),41 CD44-positive patients(43.16%) and 56 CD24-positive patients(58.95%).Thirty patients were both EPCAM and CD44 positive(31.58%),45 patients were both EPCAM and CD24 positive(47.37%),32 patients were both CD44 and CD24 positive(33.68%),and 25 patients were EPCAM,CD44 and CD24 positive(26.32%).EPCAM expression was correlated with age,depth of tumor infiltration and WHO histological classification.CD44 expression was correlated with BORRMANN and WHO histological classification as well as CEA value.CD24 expression was correlated with the depth of infiltration,location of the tumor,WHO histological classification and viscera invasion.All positive expression of EPCAM,CD44 and CD24 was correlated with the depth of infiltration,location of the tumor and WHO histological classification(P<0.05).The difference of survival rate between EPCAM positive group and negative group was observed,and the CD44 positive group and negative group had the same result(P<0.05 and P<0.01,respectively).The difference of survival rate between EPCAM+CD44+CD24+ group and EPCAM-CD44-CD24-group was statistically significant(P<0.05).The difference of survival rate between EPCAM-CD44+CD24+ group and EPCAM-CD44-CD24-group was also significant(P<0.05).CONCLUSION: The positive rates of EPCAM,CD44 and CD24 expression are high in gastric cancer tissues and these 3 proteins can be used as primary screening targets.