miR-18a对人主动脉内皮细胞血管生成能力的影响

目的: 观察miR-18a对人主动脉内皮细胞血管生成能力的影响。方法: 分别将10 nmol/L miR-18a mimics和20 nmol/L miR-18a inhibitor转染至人主动脉内皮细胞,qRT-PCR法检测miR-18a表达情况并观察内皮细胞的迁移、黏附和管腔形成能力。结果: 转染48 h后,miR-18a mimics组miR-18a表达为对照组的608倍(P<0.05);miR-18a inhibitor组miR-18a表达降低至 对照组的31%(P<0.05)。与对照组相比,miR-18a mimics转染后迁移至Transwell小室下层的内皮细胞数目和毛细血管管腔形成数目分别减少60%和52%(P<0.01);而miR-18a inhibitor转染后分别增加100%(P<0.05)和84%(P<0.01),细胞黏附能力增加43%(P<0.05)。结论: miR-18a的表达水平与人主动脉内皮细胞的血管生成能力相关,可能成为血管性疾病治疗的分子靶点。

Effect of miR-18a on angiogenesis of human aortic endothelial cells

AIM: To study the effect of miR-18a on angiogenesis of human aortic artery endothelial cells. METHODS: After 10 nmol/L miR-18a mimics or 20 nmol/L inhibitor was transfected into human aortic endothelial cells, the expression level of miR-18a was determined by qRT-PCR, and the capacities of endothelial cell angiogenesis, such as migration, adhesion and tube formation, were observed. RESULTS: Forty-eight hours after transfection with miR-18a mimics, the expression of miR-18a was as 608 folds as the control (P<0.05), but decreased to 31% of the control level in miR-18a inhibitor transfection group (P<0.05). Compared with control group, the numbers of endothelial cells, which migrated to the lower Transwell chamber and formed capillary-like tubes, declined by 60% and 52%, respectively, in miR-18a mimics group (P<0.01), and they increased by 100% and 84%, respectively, in miR-18a inhibitor transfection group (P<0.05, P<0.01). In addition, the inhibitor treatment group displayed more potent adhesion capacity, 43% higher than that in control group (P<0.05). CONCLUSION: miR-18a is involved in angiogenesis of human arterial endothelial cells, and might be a potential molecular therapeutic target of vascular diseases.