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| 肝素涂层医用聚氯乙烯的细胞毒性研究 | |
| 引用本文： | 杨剑,易定华,刘金成,刘剑,曹瑞军,秦锋. 肝素涂层医用聚氯乙烯的细胞毒性研究[J]. 生物医学工程学杂志, 2005, 22(4): 739-741 |
| 作者姓名： | 杨剑 易定华 刘金成 刘剑 曹瑞军 秦锋 |
| 作者单位： | 1. 第四军医大学,西京医院,心脏外科,西安,710033 2. 西安交通大学,应用化学系,西安,710049 3. 总参管理局,中心门诊部,北京,100088 |
| 摘 要： | 本研究通过肝素—氯烃基二甲基代苯甲胺复合物(A组)、肝素—苯扎溴胺复合物(B组)和肝素—聚乙烯亚胺复合物对医用聚氯乙烯体外循环管道进行了肝素涂层,同时采用材料直接接触细胞培养法和四唑盐(MTT)比色试验评价各种涂层材料的细胞毒性。结果显示A、B组材料直接接触L-929细胞后24h即引起细胞大量死亡,而C组材料直接接触后具有良好的细胞相容性。MTT比色试验也表明A、B两种涂层材料具有较低的OD值。A、B两种涂层材料具有较强的细胞毒性,而C方法处理的聚氯乙烯材料不引起细胞死亡,具有进一步临床应用前景。 |
| 关 键 词： | 肝素涂层 体外循环 细胞毒性 |
| 收稿时间： | 2003-04-21 |
| 修稿时间： | 2003-04-212003-10-21 |
Cytotoxicity Study of Heparin Coated Polyvinyl Chloride Material |
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| Yang Jian,YI Dinghua,LIU Jincheng,Liu Jian,Cao Ruijun,Qin Feng. Cytotoxicity Study of Heparin Coated Polyvinyl Chloride Material[J]. Journal of biomedical engineering, 2005, 22(4): 739-741 | |
| Authors: | Yang Jian YI Dinghua LIU Jincheng Liu Jian Cao Ruijun Qin Feng |
| Affiliation: | Center of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China. yangjian1212@hotmail.com |
| Abstract: | In this study, heparin ionically coated polyvinyl chloride (PVC) material was prepared by heparin-benzalkonium chloride complex (Group A), heparin-benzalkonium bromide complex (Group B) and heparin-polyethyleneimine compound (Group C). Cytotoxicity evaluation was conducted by direct cell contact evaluation and MTT colorimetry method. The results showed Group A and Group B caused L-929 cells to die out while Group C showed good compatibility with cells. The OD levels of Group A and B were lower than that of the Group C in MTT test. Method A and method B of heparin coating had remarkable cytotoxicity, while method C had little cytotoxicity and could be further studied for clinical use. |
| Keywords: | Heparin coating Cardiopulmonary bypass Cytotoxicity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |