Solid surface chemical and physical effects on the adsorption of recombinant Factor VIII |
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Authors: | Tai Yuan-Ching McGuire Joseph Joshi Omkar Wang D Q |
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Affiliation: | Department of Chemical Engineering, Oregon State University, Corvallis, OR 97331, USA. |
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Abstract: | The adsorption of a recombinant Factor VIII to silica surfaces coated to present different hydrophilic/hydrophobic, electrostatic and steric characteristics was monitored in situ, with ellipsometry. rFVIII adsorption affinity was high for hydrophobic surfaces and hydrophilic, charged surfaces. A lower affinity was recorded between rFVIII and a neutral hydrophilic surface, with substantial reduction in rFVIII adsorption to electronically neutral surfaces presenting pendant poly[ethylene oxide] chains. We conclude that steric repulsion is a requirement for minimizing rFVIII adsorption at solid surfaces. |
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