全氟异丁烯对小鼠肺组织巯基的影响

目的 观察全氟异丁烯（perfluoroisobutylene，PFIB）吸入性急性肺损伤（acute lung injury，ALI）发病过程中，肺组织内巯基的变化情况。方法 采用动态吸入PFIB周身暴露装置染毒小鼠，Ellman试剂法检测PFIB染毒前后小鼠肺组织内巯基（总巯基、蛋白巯基和非蛋白巯基）的含量变化。结果 染毒后，小鼠肺组织中非蛋白巯基（主要是还原型谷胱甘肽）含量与正常动物相比显著性的逐渐升高。结论 氧化性损伤可能不是PFIB吸入性肺损伤发病过程中主要的致病因素。

Effects of inhaled perfluoroisobutylene on sulfhydryls in the lung tissues of mice

Objective To investigate the changes of sulfhydryls(SH)in acute lung injury(ALI)in mice induced by inhalation of perfluoroisobutylene(PFIB).Method Mice were exposed to gaseous PFIB for 5 minutes in a flow-past whole-body exposure system.The changes of total sulfhydryls(TSH),nonprotein sulfhydryl(NPSH)and protein sulfhydryl(PSH)in the lung tissues were assayed by Ellman's reagent method.Result Level of NPSH,mainly reduced gluthathion,elevated significantly in the lung tissues of mice from 4h to 24h after ALI induced by PFIB inhalation,as compared to that in normal mice.Conclusion Oxidative damage may not play a pivotal role in mouse ALI induced by PFIB inhalation.