| IRM-2小鼠胚胎成纤维细胞的原代培养和生物学特性 |
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| 引用本文： | 王芹,李进,张恒,宋力,刘强,岳井银,樊飞跃. IRM-2小鼠胚胎成纤维细胞的原代培养和生物学特性[J]. 苏州大学学报(自然科学版), 2010, 30(1): 1-3,22,F0002 |
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| 作者姓名： | 王芹 李进 张恒 宋力 刘强 岳井银 樊飞跃 |
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| 作者单位： | 中国医学科学院放射医学研究所生物室天津市分子核医学重点实验室; |
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| 基金项目： | 国家自然科学基金资助项目(30870583); 中国医学科学院放射医学研究所学科发展基金资助项目(SF0822) |
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| 摘 要： | 目的分离原代培养IRM-2小鼠胚胎成纤维细胞（MEFs）,从本质上揭示IRM-2小鼠的辐射抗性。方法分离IRM-2小鼠MEFs,并进行原代培养和传代培养。观察MEFs生长形态,测定第3代和第5代细胞生长曲线、细胞周期和增殖指数。结果IRM-2小鼠在胎龄14～16d进行原代MEFs分离,MEFs在体外为贴壁生长型细胞,6代前MEFs生长良好。第3代和第5代MEFs在培养至第3天开始进入对数生长期,到第6天时细胞数达到高峰。MEFs增殖活跃,第3代和第5代细胞增殖指数分别为53%和46%。结论成功分离、培养了IRM-2小鼠MEFs,为进一步利用MEFs研究小鼠的辐射抗性机制奠定了基础。
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| 关 键 词： | 小鼠胚胎成纤维细胞 IRM-2小鼠 原代培养 |
Culture of IRM-2 Mouse Embryonic Fibroblasts and Biological Character |
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| WANG Qin,LI Jin,ZHANG Heng,SONG Li,LIU Qiang,YUE Jing-yin,FAN Fei-yue. Culture of IRM-2 Mouse Embryonic Fibroblasts and Biological Character[J]. Suzhou University Journal of Medical Science, 2010, 30(1): 1-3,22,F0002 |
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| Authors: | WANG Qin LI Jin ZHANG Heng SONG Li LIU Qiang YUE Jing-yin FAN Fei-yue |
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| Affiliation: | WANG Qin,LI Jin,ZHANG Heng,SONG Li,LIU Qiang,YUE Jing-yin,FAN Fei-yue(Dept of Biology,Institute of Radiation Medicine,Chinese Academy of Medical Sciences,Tianjin Key Laboratory of Molecular Nuclear Medicine,Tianjin 300192,China) |
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| Abstract: | Objective To isolate,cultivate the primary mouse embryonic fibroblasts(MEFs)derived from IRM-2 mice and identify their inherent radioresistance character.Methods MEFs were isolated.MEFs cultured were passed on from generation to generation.MEFs of passage 3 and 5 were assessed by cell morphology,growth curves and cell cycles.Results Primary MEFs were isolated from 14 to 16 day pregnant IRM-2 mice.MEFs in vitro were a kind of adherent cells with good growth ability prior to passage 6.MEFs of passage 3 and 5 ... |
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| Keywords: | mouse embryonic fibroblasts IRM-2 mice primary generation culture |
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