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| 1-磷酸鞘氨醇对淋巴管内皮细胞生物学特性调节作用的研究 | |
| 引用本文： | 肖秀斌,王华,李庆芳,严俊,杨月峰,徐尹,张伟京,王立生.1-磷酸鞘氨醇对淋巴管内皮细胞生物学特性调节作用的研究[J].军事医学科学院院刊,2009,33(4):326-329. |
| 作者姓名： | 肖秀斌 王华 李庆芳 严俊 杨月峰 徐尹 张伟京 王立生 |
| 作者单位： | 1. 军事医学科学院附属医院肿瘤科,北京,100071 2. 军事医学科学院放射与辐射医学研究所,北京,100850 3. 军事医学科学院放射与辐射医学研究所,北京,100850;安徽医科大学生物化学与分子生物学教研室,合肥,230032 |
| 基金项目： | 国家"973"课题，国家自然科学基金资助项目 |
| 摘 要： | 目的：研究1-磷酸鞘氨醇（S1P）对淋巴管内皮细胞（HDLEC）的生物学特性的调节作用及其调节机制。方法：应用RT-PCR方法检测HDLEC是否表达SPK及S1P受体,应用MTT法、细胞扩散盒技术检测S1P对HDLEC增殖、迁移特性的影响,并用Western印迹方法检测S1P对HDLEC的MAPK信号通路的调节。结果：HDLEC表达SPK及S1P受体,包括S1P1,S1P2,S1P3和S1P4。外源性S1P对HDLEC没有促增殖作用,但可促进其迁移,并可通过S1P1和S1P2诱导MAPK快速磷酸化。结论：初步研究结果提示,S1P可以影响淋巴管内皮细胞的生物学特性,有可能成为调控淋巴管生成及其功能的新靶点。 |
| 关 键 词： | 1-磷酸鞘氨醇 1-磷酸鞘氨醇受体 淋巴管内皮细胞 细胞增殖 迁移 |
Effects of sphingosine-1-phosphate on functions of human lymphatic endothelial cells |
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| XIAO Xiu-Bin,WANG Hua,LI Qing-Fang,YAN Jun,YANG Yue-Feng,XU Yin,ZHANG Wei-Jing,WANG Li-Sheng.Effects of sphingosine-1-phosphate on functions of human lymphatic endothelial cells[J].Bulletin of the Academy of Military Medical Sciences,2009,33(4):326-329. | |
| Authors: | XIAO Xiu-Bin WANG Hua LI Qing-Fang YAN Jun YANG Yue-Feng XU Yin ZHANG Wei-Jing WANG Li-Sheng |
| Institution: | XIAO Xiu-Bin, WANG Hua, LI Qing-Fang, YAN Jun, YANG Yue-Feng, XU Yin, ZHANG Wei-Jing, WANG Li-Sheng (1. Department of Oncology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China;2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China; 3. Department of Biochemistry and Molecular Biology,Anhui Medical University,Hefei 230032 ,China) |
| Abstract: | Objective：To investigate the possible effects of sphingosine-l-phosphate （S1P） on functions of human lym- phatic endothelial cells （HDLEC） and the mechanisms. Methods： RT-PCR was used to detect the expression of sphingosine kinase （SPK） and S1P receptors on HDLEC. The proliferation of HDLEC was determined by MTY, and the migration of HDLEC was observed by Transwell technique. Finally, Western blot was used to test the effect of S1P on MAPK phosphoryl- ation. Results：HDLEC expressed SPK and S1P receptors, including S1P1 ,S1P2 ,S1P3and S1P4. Exogenous SIP could pro- mote the migration of HDLEC, but not the proliferation in vitro, and could induce rapidly the phosphorylation of MAPK through S1P receptor subtypes S1P1 and SIP2 of HDLECs. Conclusion：The results suggest that SIP and its receptors can serve as potential new therapeutic targets in lymphatic vessel formation. |
| Keywords: | sphingosine-1-phosphate sphingosine-1-phosphate receptor human lymphatic endothelial cells cell proliferation migration |
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