| 食品中花粉红染料的测定 |
| |
| 引用本文： | 邵仕萍,奚星林. 食品中花粉红染料的测定[J]. 中国卫生检验杂志, 2010, 0(1) |
| |
| 作者姓名： | 邵仕萍 奚星林 |
| |
| 作者单位： | 广东出入境检验检疫局技术中心食品实验室; |
| |
| 摘 要： | 目的:建立测定食品中花粉红染料的高效液相色谱方法。方法:样品经磷酸水溶液或乙腈-磷酸水溶液提取,Sep-Pak C18固相萃取柱净化。采用ODS柱分离,以甲醇和水溶液流动相进行洗脱,在激发波长550 nm,发射波长580 nm的荧光条件下检测。结果:花粉红的质量浓度在0.5~15.0μg/L范围内与其色谱峰面积的线性关系良好(r=0.999),在1、5、10μg/kg添加水平时的回收率为50.8%~83.5%,RSD为2.1%~7.5%。方法的定量检出限为1.0μg/kg。结论:该方法准确度高,分离效能好,结果稳定可靠,适合于食品中花粉红含量的测定。
|
| 关 键 词： | 高效液相色谱法 花粉红 食品 测定 |
Determination of rhodamine B dyestuff in foods |
| |
| SHAO Shi-ping,XI Xing-lin. Determination of rhodamine B dyestuff in foods[J]. Chinses Journal of Health Laboratory Technology, 2010, 0(1) |
| |
| Authors: | SHAO Shi-ping XI Xing-lin |
| |
| Affiliation: | SHAO Shi-ping,XI Xing-lin (Guangdong Entry-Exit Inspection , Quarantine Technology Centre,Guangzhou 510623,China) |
| |
| Abstract: | Objective:To develop a method for the quantitative analysis of rhodamine B dyestuff in foods.Methods:The sample which was dissolved in phosphoric acid solution or acetonitrile-phosphoric acid solution was extracted by adsorption with Sep-Pak C18 column.HPLC was carried out on the ODS column with the fluorescent specrometer and methyl alocohol-water solution as mobile phase.The exciation wavelength is 550 nm.The emission wavelength is 580 nm.Results:There was good linear relationship between the peak area an... |
| |
| Keywords: | HPLC Rhodamine B Food Determination |
| 本文献已被 CNKI 等数据库收录！ |
|
