河南贲门癌高发区贲门癌的组织化学染色分类及P53和PCNA研究

目的探讨贲门腺癌(Gastriccardia adenocarcinoma,GCA)的组织化学染色分类与P53和PCNA分子学特征。方法采用爱显蓝-雪夫试剂染色法(Alcianblue-Periodicacid Schiff,AB-PAS)和高铁二胺-爱显蓝染色法(High Iron Diamine-Alcianblue,HID-AB)以及ABC免疫组织化学分析(Immunohistochemistry,IHC)法研究了70例贲门癌的组织化学及P53和PCNA蛋白表达变化特征。结果肠型GCA的P53蛋白阳性表达(+~)率(73.08%)明显高于非肠型GCA(29.55%)(P<0.01);PCNA蛋白阳性高表达(~)率(96.15%)也明显高于非肠型GCA(65.91%)(P<0.01)。含硫化粘蛋白的GCA组织中P53蛋白阳性表达(+~)率(70.59%)明显高于不含硫化粘蛋白的GCA(41.51%)(P<0.05);PCNA蛋白阳性高表达(~)率(94.12%)也明显高于不含硫化粘蛋白的GCA(71.70%)(P<0.05)。结论组织化学染色可作为判断GCA不同组织类型和分类的重要指标,并可能对了解GCA临床生物学特征(如预后)具有重要意义。

The studies of histochemistry staining patterns and P53,PCNA on gastric cardia adenocarcinoma (GCA) from patients at high-incidence area for GCA in Henan

Objective: To characterize the histochemistry and expression of P53,PCNA proteins on gastric cardia adenocarcinoma (GCA) .Methods: The methods of Histochemical (AB-PAS and HID-AB methods) and immunohistochemical analysis (ABC method) were performed on the 70 cases of surgically resected tissues with GCA . Results: The positive expression rate for P53 protein (+～ ) in intestinal type GCA (73.08%) was significantly higher than that in non-intestinal type GCA (27.27%,P<0.01), and the high positive expression rate of PCNA protein (～) in intestinal type GCA (96.15%) was significantly higher than that in non-intestinal type GCA (65.91%,P<0.01). The positive expression rate for P53 protein (+～) in sulfuration type (70.59%) was significantly higher than that in non-sulfuration type (41.51%,P<0.05), and the high positive expression rate of PCNA protein (～) in sulfuration type (94.12%) was significantly higher than that in non-sulfuration type (71.70%,P<0.05).Conclusion: The positive expression rate for P53 (+～ ) and PCNA (～ ) in intestinal type GCA were significantly higher than those in non-intestinal type GCA, and in sulfuration type GCA were significantly higher than that in non-sulfuration type GCA, which indicated that histochemical staining may be one of promising biomarkers for GCA classification and histotype, possibly for further understanding of the clinical behavior of GCA (e.g.prognosis).