MMP-2、MMP-9在大鼠皮肤创面愈合过程中的表达

目的探讨基质金属蛋白酶2(MMP-2)、MMP-9在大鼠皮肤创面愈合过程中表达的动态及其相关性。方法 42只大鼠制作大鼠背部皮肤创面模型后按取创面皮肤时间随机均分为七组,即在创面形成后1、12、24h及3、7、14、21d切取背部皮肤创面标本,采用免疫组化法检测标本中MMP-2及MMP-9的表达。另取3只正常大鼠背部皮肤标本作为对照。结果正常大鼠皮肤中MMP-2、MMP-9几无表达;背部皮肤创面形成后,MMP-2逐渐升高,14d达峰,21d仍高于正常水平;MMP-9迅速升高,在24h-7d呈高水平表达,后逐渐降至较低水平;MMP-2与MMP-9的表达水平在12h,3、7、14、21d时呈正相关,在24h时呈负相关。结论在大鼠创面愈合过程中MMP-2和MMP-9的表达有一定规律,并且二者相互影响。

Expressions of MMP-2 and MMP-9 during wound healing in rat skin

Objective To investigate dynamic changes of metalloproteinase-2(MMP-2) and MMP-9 expressions during wound healing in rat skin and their correlation.Methods Forty-two rats were used to establish dorsal skin wounds model and equally randomized into seven groups according to the times when the biopsies from skin wounds were harvested at 1,12,24 hours and 3,7,14,21 days after wounds formation,respectively.The other 3 rats were taken as the controls.The expressions of MMP-2 and MMP-9 in the samples were detected by immunohistochemistry.ResultsMMP-2 and MMP-9 were hardly expressed in normal skins of the control rats.The expression of MMP-2 increased gradually after injury and reached the peak on the 14th day,then decreased gradually to a certain level.The expression of MMP-9 increased rapidly and showed a high level from 24 hours to 7 days after injury,then decreased gradually to a low level.The expression level of MMP-2 was positively correlated to that of MMP-9 at 12 hours and 3,7,14,21 days and negatively correlated at 24 hours after injury.Conclusion The MMP-2 and MMP-9 expressions have certain rule and influence each other during wound healing in rats.