二巯基丁二酸修饰的Fe3O4纳米磁液联合碘油动脉栓塞热疗治疗兔VX2肝癌

目的评估二巯基丁二酸修饰的Fe3O4(DSMA-Fe3O4)纳米磁液联合碘油动脉栓塞热疗治疗兔VX2肝癌的疗效。方法 25只开腹肝左叶种植VX2瘤的实验兔,随机等分为五组:对照组(A组)、碘油栓塞组(B组)、DSMA-Fe3O4纳米磁液+碘油栓塞组(C组)、DSMA-Fe3O4纳米磁液+热疗组(D组)、DSMA-Fe3O4纳米磁液+碘油栓塞+热疗组(E组)。成瘤2周后各组行CT扫描并测量,随后行肝动脉栓塞,D、E组栓塞后诱导热疗。分别在术后7、14d行CT检查,计算肿瘤生长比率、肿瘤增长体积。14dCT扫描结束后每组处死部分实验兔,取完整的肝、肾、脾及肺作病理检查。各组分别在术前1d,术后1、3、7d经兔耳缘静脉采血测ALT和AST。结果所有肝癌模型均建立成功。五组术前肿瘤体积、术前1d、术后7dALT、AST水平均无统计学差异(P>0.05)。术后14d,E组肿瘤体积平均缩小26.7%,而B、C、D三组肿瘤体积分别平均增大了200.7%、209.4%和422.5%。结论 DSMA-Fe3O4纳米磁液联合碘油动脉栓塞热疗兔VX2肝癌有效、安全。

Treating the experimental rabbit VX2 hepatic tumor with arterial embolization hyperthermia with DSMA-Fe3O4 nano-sized magnetic fluid and lipiodol

Objective To evaluate the efficacy of treating the experimental rabbit VX2 hepatic tumor with arterial embolization hyperthermia with DSMA-Fe3O4 nano-sized magnetic fluid and lipiodol. Methods Twenty-five rabbits were implanted with VX2 hepatic tumors into the left hepatic lobes.Then they were randomly divided into five groups of A(control),B(lipiodol embolization),C(combined use of DSMA-Fe3O4 nano-sized magnetic fluid and lipiodol embolization),D(DSMA-Fe3O4 nano-sized magnetic fluid embolization hyperthermia), and E(combined use of DSMA-Fe3O4 nano-sized magnetic fluid and lipiodol embolization hyperthermia).The volume of the xenografts was measured by CT scanning on the 14th day.The transarterial embolization was conducted with different emboliaztion agents mentioned above.The rabbits in groups of D and E accepted hyperthermia after embolization. The volume of the xenografts was followed-up by CT scanning on the 7th and 14th day after treatment.Then some rabbits of each group were sacrificed and the liver,spleen,kidney and lungs were removed for histopathology examination.Meanwhile,alanine aminotransferase(ALT) and aspartate aminotransaminase(AST) of all rbbits were measured one day before ernbolization and on the 1st,3rd and 7th day after therapy,respectively. Results All rabbit models with VX2 hepatic xenografts were successfully established.The differences in the tumor volumes,ALT and AST levels before treatment and on the 7th day after treatment were not statistically significant among 5 groups. On the 14th day after treatment,the tumor size in group E was decreased by 26.7% compared to that before treatment,which in groups of B,C and D was increased by 200.7%, 209.4% and 422.5%,respectively. Conclusion Arterial embolization hyperthermia with a combination of DSMA-Fe3O4 nano-sized magnetic fluid and lipiodol is effective,feasible and safe in treating VX2 hepatic xenografts in rabbits.