电刺激对鼠坐骨神经横断后脊髓运动神经元凋亡的影响

目的 观察直流电场对鼠坐骨神经横断后脊髓运动神经元凋亡的影响。方法 鼠坐骨神经横断后，实验组在相应的脊髓节段立即置入直流电刺激器，对照组置入无输出电流的刺激器，分掇于术后1、4、7、14、28d取材，应用HE染色，光镜下进行形态学观察，对脊髓运动神经元计数半进行立体定量分析，用TUNEL染色，对脊髓运动神经元中阳性细胞计数。结果 坐骨社会横断后4、7、14、28d，实验组脊髓运动神经元数量明显多于对照组，术后7、14、28d，实验组脊髓运动神经元体积在于对照组，运动神经元TUNEL染色阳性数明显小于对照组。结论 直流电刺激对坐骨神经横断引起的脊髓运动神经元凋亡具有防治作用。

The effects of direct-current field stim ulation on spinal cord motor neuron apo ptosis after sciatic nerve transection I n rats

Objective To study the effects of direct-current field stimulation on spinal cord motor neurons apoptosis after sciatic nerves transection Methods In experimental group direct-current stimulators were put into the relevant spinal cord segments and electric stimulation were given instantly after sciatic nerve transectionIn control group, the same stimulator were put inside without courrent outputThe spinal cord specimens were taken 1,4,7,14,28 days after operationThe morphology of the spinal cord motor neurons was observed with the methods of HE staining and light microscopeThe spinal cord motor neurons were counted and analyzed with three-dimensional quantitative methodThe TUNEL staining of the specimens was performed simultaneously and the positive-stain spinal cord motor neurons were counted Results 4,7,14 and 28 days after operation, the number of spinal cord motor neurons and the size of the neurons increased obviously with compare to the control groupThe TUNEL staining positive spinalcord motor neurons decreased significantly according to the control group Conclusion Direct-current stimulation may have intervention effects on spinal cord neuron apoptosis after sciatic nerve transection