| HPLC法测定盐酸三氟拉嗪含量及有关物质 |
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| 引用本文： | 张君,廖声华,严拯宇. HPLC法测定盐酸三氟拉嗪含量及有关物质[J]. 海峡药学, 2010, 22(3): 43-45 |
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| 作者姓名： | 张君 廖声华 严拯宇 |
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| 作者单位： | 中国药科大学分析化学教研室,南京,211198;中国药科大学分析化学教研室,南京,211198;中国药科大学分析化学教研室,南京,211198 |
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| 摘 要： | 目的建立盐酸三氟拉嗪含量测定及有关物质限量检查的HPLC法。方法采用C18柱,以乙腈-磷酸盐缓冲溶液（0.05mol·L^-1NaH2PO4.2H2O100mL,加2mL的三乙胺后用磷酸调节pH至3.0）（38∶62）为流动相。流速：1.0mL·min^-1,柱温：30℃,检测波长：258nm。结果盐酸三氟拉嗪与其他杂质能较好分离,盐酸三氟拉嗪浓度在2.5～25μg·m^L-1范围内线性关系良好（r=0.9997）,检测限为0.016μg·mL^-1。结论本方法灵敏、准确、专属性好,可用于盐酸三氟拉嗪的含量测定及有关物质限量检查。
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| 关 键 词： | 盐酸三氟拉嗪 高效液相色谱法 含量测定 有关物质 |
Determination of trifluoperazine hydrochloride and its related substances by HPLC |
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| ZHANG Jun,LIAO Sheng-hua,YAN Zheng-yu. Determination of trifluoperazine hydrochloride and its related substances by HPLC[J]. Strait Pharmaceutical Journal, 2010, 22(3): 43-45 |
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| Authors: | ZHANG Jun LIAO Sheng-hua YAN Zheng-yu |
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| Affiliation: | (China Pharmaceutical University,Nanjing 211198,China) |
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| Abstract: | OBJECTIVE To establish HPLC methods for the determination of trifiuoperazine hydrochloride and its related substances.Methods C18 column was used,the mobile phase wasacetonitrile-phosphate buffered solution（0.05 mol·L^-1 NaH2PO4·2H2O 100mL,add 2 mLtriethylamine,and adjusted to pH 3.0 with phosphoric acid）（38∶62）.The flow rate was 1.0ml·min^-1,the column temperature was 30℃ and the detection wavelength of UV detector was258 nm.RESULTS trifluoperazine hydrochloride was separated completely from impurities.Thelinear range of trifluoperazine hydrochloride was 2.5～25μg·mL^-1（r=0.9997）.The limit of quantitation was 0.016ug·mL^-1.CONCLUSION With good sensitivity,accuracy,and selectivity,the method can be applicable for the determination of trifiuoperazine hydrochloride and its related substances. |
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| Keywords: | trifiuoperazine hydrochloride HPLC quantification related substances |
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