小鼠代谢性高尿酸血症模型的复制方法初探

目的本实验分别单独使用或联合使用次黄嘌呤、尿酸氧化酶抑制剂复制小鼠代谢性高尿酸血症模型，观察造模后受试动物血清尿酸值的变化，以确定次黄嘌呤、尿酸氧化酶抑制剂造模的最佳方式、给药途径和剂量，并通过阳性药验证该模型的可行性。方法采用次黄嘌呤灌胃或腹腔注射给药，尿酸氧化酶抑制剂腹腔或皮下注射给药，分别测定造模后不同时段小鼠血尿酸值。结果复制小鼠高尿酸血症模型，采用腹腔注射次黄嘌呤500mg／kg，皮下注射尿酸氧化酶抑制剂50mg／kg，1、3、5h后，血尿酸值明显高于正常组（P〈0．01），且阳性药可显著降低升高的尿酸值（P〈0．01）。结论腹腔注射次黄嘌呤500mg／kg和皮下注射尿酸氧化酶抑制剂50mg／kg配合使用，是复制小鼠高尿酸血症模型的适当组合。

A Preliminary Study on the Establishment of a Mouse Model of Hyperuricemia

Objective To investigate the effects of hypoxanthine and uricase inhibitor used singly or in combination on the concentration of serum uric acid in mice,to determine the optimal procedure,route and dosage of drug administration,and to evaluate the feasibility of this method to establish a mouse model of hyperuricemia.Methods After i.g.or i.p administration of hypoxanthine and i.p.or s.c.injection of uricase inhibitor,the concentration of serum uric acid was measured at different time intervals.Results At 1,3,5 hours after i.p.injection of hypoxanthine in a dose of 500 mg/kg and s.c.injection of uricase inhibitor in a dose of 50 mg/kg,the level of serum uric acid in mice was significantly increased(P<0.01).Moreover,positively reacting drugs can significantly reduce the increased serum uric acid in those mice(P<0.01).Conclusion Intraperitoneal injection of hypoxanthine in a dose of 500 mg/kg and subcutaneous injection of uricase inhibitor in a dose of 50 mg/kg,administered in an appropriate combination,is a reliable method to induce significant increase of serum uric acid in mice and can be used as a suitable method to establish a mouse model of hyperuricemia.