Prenatal administration of indomethacin modulates Th2 cytokines in juvenile rats |
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| Authors: | Kiyoshi Kushima Shozo Sakuma Shuichi Furusawa Michio Fujiwara |
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| Institution: | 1. Drug Safety Research Laboratories, Astellas Pharma Inc., 1-6, Kashima 2-chome, Yodogawa-ku, Osaka 532-8514, Japan;2. Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan |
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| Abstract: | Indomethacin (IND) suppresses the T-dependent antibody response (TDAR) in juvenile males when it is administered to pregnant rats during late gestation. In this study, the effect of IND on cytokine production in juvenile rats was examined to investigate the mechanism behind the suppression of antibody production. IND was orally administered to pregnant SD rats on days 18–21 of gestation. After parturition, the spleen cells isolated from 3-week-old pups were incubated with concanavalin A (Con A) or lipopolysaccharide (LPS). The level of cytokines in the culture supernatant was measured. IL-10 decreased significantly in the males, and IL-6 and TNF-α tended to decrease in both sexes. |
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| Keywords: | Developmental immunotoxicity Indomethacin Rat Th1/Th2 cytokines Mitogenic stimulation |
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