豆根管食通口服液对食管癌造模大鼠细胞周期调控因子的影响

目的观察豆根管食通口服液对食管癌造模大鼠细胞周期调控因子的影响,从细胞分子水平探讨该药的作用机制。方法选用健康Wistar大鼠,随机分为正常组,模型组,豆根管食通高、中、低剂量组,化疗组,应用S-P免疫组化法检测大鼠食管组织p16、Rb、cyclinD1、CDK4的表达。结果各药物治疗组p16、Rb表达率均有不同程度的升高,以豆根管食通高剂量组升高最为明显,与模型组比较有统计学意义(P<0.05)。cyclinD1、CDK4在食管癌模型组表达率明显提高,与正常组比较有显著性差异(P<0.01)。结论豆根管食通口服液对食管癌具有较好的防治作用,其抗癌作用的分子机理可能是通过上调抑癌基因p16、Rb,下调癌基因cyclinD1、CDK4的表达,阻断细胞由G1期向S期过渡,从而抑制大鼠食管癌细胞生长。

The Effect of Dougenguanshitong Oral Liquids on the Cell Cycle Regulation Factor of Modeled Esophageal Carcinoma Rats

Objective To observe the effect of Dougenguanshitong (DGGST) oral liquids on the cell cycle regulation factor of modeled esophageal carcinoma rats, explore the function mechanism of the medicine at molecular level. Methods Healthy Wistar rats were divided into the following groups - esophageal carcinoma group, DGGST high dose group, middle dose group, low dose group, the Tegafur tablets group. To examine the immunohistochemical staining of p16, Rb, cyclin D1, CDK4, PCNA. Results After treatment, the expression of p16 and Rb increased, evidently in the DGGST high dose group. The results were significant (P <0.05). After treatment, cyclin D1, CDK4 increased especially in the DGGST high dose group and the chemotherapy group, compared with the model group (P <0.05). Conclusion DGGST has good prevention and treatment effect on esophageal carcinoma. The molecular mechanism of DGGST resisting esophageal carcinoma may by increasing p16, Rb and decreasing cyclin D1 and CDK4, and stopping the process of cell cycle from G1 to S phase.