| 苦参碱诱导人乳腺癌细胞MCF-7/ADR的凋亡研究 |
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| 引用本文： | 周炳刚,孙靖中,苏刚,马德强. 苦参碱诱导人乳腺癌细胞MCF-7/ADR的凋亡研究[J]. 中华实验外科杂志, 2003, 20(6): 515-516 |
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| 作者姓名： | 周炳刚 孙靖中 苏刚 马德强 |
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| 作者单位： | 1. 250012,济南,山东大学齐鲁医院普外科
2. 宁夏自治区人民医院普外科 |
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| 摘 要： | 目的 研究苦参碱对人乳腺癌MCF—7／ADR细胞的生长抑制作用及诱导凋亡机制。方法 四甲基噻唑蓝(MTT)法测定苦参碱对细胞的半数抑制浓度(IC50)，用荧光显微镜、电子显微镜、激光共聚焦显微镜、流式细胞仪和DNA凝胶电泳等技术观察细胞凋亡。结果 0．3—2．5g／L浓度苦参碱对MCF—7／ADR细胞有明显的抑制作用，并诱导发生凋亡，实验范围内细胞凋亡的比率与药物浓度呈一定相关。结论 苦参碱能抑制人乳腺癌MCF—7／ADR细胞的生长，其机制与阻止细胞周期的进程，启动细胞自身调控程序，诱导凋亡有关。
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| 关 键 词： | 苦参碱 人乳腺癌细胞 MCF—7／ADR 细胞凋亡 乳腺癌 |
| 修稿时间： | 2002-09-05 |
Apoptosis of human breast cancer MCF-7/ADR cells induced by matrine |
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| ZHOU Bing-gang ,SUN Jing-zhong,SU Gang,et al.. Apoptosis of human breast cancer MCF-7/ADR cells induced by matrine[J]. Chinese Journal of Experimental Surgery, 2003, 20(6): 515-516 |
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| Authors: | ZHOU Bing-gang SUN Jing-zhong SU Gang et al. |
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| Affiliation: | ZHOU Bing-gang *,SUN Jing-zhong,SU Gang,et al. *Department of General Surgery,Qilu Hospital of Shandong University,Jinan 250012,China |
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| Abstract: | Objective To investigate the growth-inhibition and the mechanism of apoptosis induced by matrine on MCF-7/ADR cell line.Methods Using MTT assay,the half-inhibition concentration (IC 50 ) of marine on MCF-7/ADR cells was detected.Fluorescence microscopy,electron microscopy,laser scanning confocal microscopy,flow cytometry (FCM) and DNA gel electrophoresis were applied to demonstrate the presence of apoptosis.Results Apoptosis was detectable in 0.3-2.5 g/L matrine-treated MCF-7/ADR cell line.The apoptosis-inducing effect of matrine was dose-dependent in the experiment range.Conclusion Matrine can induce the inhibition of MCF-7/ADR cell growth by apoptosis and the mechanism of apoptosis is related to the blocking of S period cells. |
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| Keywords: | Breast neoplasms Matrine Apoptosis |
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