喂饲克山病病区粮大白鼠心肌细胞膜通透性的变化——用硝酸镧作示踪物的电子显微镜观察

以硝酸镧作示踪物用电子显微镜观察喂饲克山病病区粮3个月的大白鼠心肌细胞膜通透性的变化。结果发现喂饲克山病病区粮的动物心肌细胞肌丝间和部分线粒体内均有镧盐沉着,而非病区粮饲养的动物镧盐颗粒只见于心肌细胞外间隙。表明喂饲克山病病区粮的大白鼠心肌肌膜和部分线粒体膜通透性增大。

Myocardial sarcolemmal membrane permeability change in rats fed on grains from Keshan disease endemic area——An electron microscopic study with lanthanumnitrate as the tracer

Sarcolemmal membrane permeability alteration of cardiac myocytes in rats fed on grains from Keshan disease endemic area was studied with electron microscopy,using La(NO3)3(La3 )as the tracer. Weanling rats of both sexes were divided into two groups (10 rats in each group) ; the experimental group was fed on grains from Keshan disease endemic area,whereas the control group on grains from non-endemic area.By the end of three months of such feeding, all rats were sacrificed and the myocardial specimens for electron microscopic observation were prepared according to the methods described by Hoffstein et al.The abnormal La3 deposition was observed between myofilaments and in some mitochondria in the rats fed on grains from Keshan disease endemic area.No obvious ultrasrructural injury could be found in the myocytes with abnormal La3 deposition. In the control group,La3 tracer was confined to the extracellular space.The result indicated that permeability of sarcolemmal membrane and some mitochondrial membrane in the rats fed on grains from Keshan disease endemic area was markedly increased, suggesting that alterations of myocardial cell membrane permeability may play an important role in the pathogenesis of the myocardial damage in Keshan disease.