初发SLE患者外周血中CD4+ T细胞表面趋化因子受体的表达及其意义

目的了解初发系统性红斑狼疮(SLE)外周血CD4+T细胞表面趋化因子受体的表达情况并探讨在发病中的意义。方法流式细胞术检测18例初发SLE患者(有肾炎10例,无肾炎8例)及10例正常对照外周血CD4+T细胞表面趋化因子受体CCR1、CCR3、CCR5的表达情况,比较SLE治疗前后以及正常对照CD4+CCR1+、CD4+CCR3+、CD4+CCR5+T细胞亚群占全部CD4+T细胞比例的差异。结果SLE患者CD4+CCR1+、CD4+CCR5+T细胞亚群百分率显著低于对照组(P均<0.001);CD4+CCR3+T细胞亚群百分率在SLE和对照组间差异无统计学意义。三种T细胞亚群在肾炎组及无肾炎组的差异均不显著(P均>0.05)。CD4+CCR1+T细胞亚群百分率治疗后显著升高(P=0.022);CD4+CCR5+T细胞亚群百分率治疗后升高(1.81±0.95%和2.88±1.42%),但无统计学意义(P=0.065);CD4+CCR3+T细胞亚群百分率治疗后变化无显著性。结论CD4+CCR1+和CD4+CCR5+T细胞亚群可能参与SLE发病机制。

The expression rate of chemokine receptors on CD4 + T cells and its pathogenic roles in new - onset systemic lupus erythematosus

Objective To explore the pathogenic roles of chemkine receptors in systemic lupus erythematosus (SLE) by detecting the expression rate of CCR1,CCR3,CCR5 on CD4+T cells.Methods The expression rate of CCR1,CCR3,CCR5 on CD4+T cells were detected by flow cytometry in 8 new-onset SLE patients (including 10 cases with nephritis and 8 without nephritis) and 10 normal controls.Results The expression rates of CCR1 and CCR5 on CD4+T cells in new-onset SLE patients were significantly lower than in controls (P<0.001);The expression rate of CCR1,CCR3,CCR5 on CD4+T cells were no different between lupus nephritis group and non-lupus nephritis group;The expression rate of CCR1 increased significantly after treatment (P=0.022), and the expression rate of CCR5 also increased (but no statistical significance were found.P=0.065).Conclusion The chemkine receptors (CCR1 and CCR5)maybe act as a important pathogenic role in SLE.