| MTT法检测医用高分子材料浸出细胞毒性的实验研究 |
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| 引用本文： | 孙成群,孟滨,崔岩,陶清晨,孙荣芳,苏怡,柴虹,李凤艳. MTT法检测医用高分子材料浸出细胞毒性的实验研究[J]. 中国公共卫生学报, 1996, 0(2) |
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| 作者姓名： | 孙成群 孟滨 崔岩 陶清晨 孙荣芳 苏怡 柴虹 李凤艳 |
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| 作者单位： | 黑龙江省卫生防疫站 |
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| 摘 要： | 介绍了在微量细胞培养板中，用四甲基偶氮唑盐（ＭＴＴ）比色法检测医用高分子材料浸出细胞毒性的方法。研究了不同细胞接种浓度下，ＭＴＴ比色法的重现性，并且在同一细胞浓度下，将此方法和细胞计数法进行了比较。结果表明，此方法操作简便，结果稳定，重复性好．
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| 关 键 词： | MTT比色法，浸出细胞毒性，医用高分子材料 |
MTT Colorimetric Assay for Lixivious Cytotoxicity of Medical Macromolecular Material |
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| Sun Chengqun,et al.. MTT Colorimetric Assay for Lixivious Cytotoxicity of Medical Macromolecular Material[J]. , 1996, 0(2) |
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| Authors: | Sun Chengqun et al. |
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| Affiliation: | Heilongjiang Provincial Health and Anti epidemic Station Harbin 150036 |
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| Abstract: | This paper described MTT Colorimatric assay for lixivious cellular toxicity of medical macromoleculat materisl in microscale cell plate. Repetitiveness of MTT Colorimetric assay under different conditions of inoculated cellular concentration was studied and this metbeds was compared with the cell counting assay in the same cellular coneehtration. The results show that this method bas the advantage of easy to operate,swiftness,stability,good repetitiveness and economhation. |
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| Keywords: | MTT Colorimetric assay Lixivious cytotoxicity Medical micomolecular material |
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