In vitro echogenicity characterization of poly[lactide-coglycolide] (plga) microparticles and preliminary in vivo ultrasound enhancement study for ultrasound contrast agent application |
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Authors: | Lavisse Sonia Paci Angelo Rouffiac Valerie Adotevi Cecile Opolon Paule Peronneau Pierre Bourget Philippe Roche Alain Perricaudet Michel Fattal Elias Lassau Nathalie |
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Institution: | Département d'Imagerie et Laboratoire d'Imagerie du Petit Animal Laboratoire, Institut Gustave Roussy, Villejuif, France. |
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Abstract: | OBJECTIVES: This work includes (1) the characterization of a reproducible polylactide-coglycolide] (PLGA) microparticle preparation with an optimial mean diameter and size distribution and (2) the preliminary in vivo ultrasonographic investigation of PLGA microparticles. METHODS: A first series of PLGA microparticle preparations (1 to 15 mum) was acoustically characterized on a hydrodynamic device to select the most appropriate for ultrasound contrast agent application. Preparations of 3-microm microparticles were selected, characterized at different doses, and then injected into 20 melanoma grafted mice for contrast-enhanced power Doppler ultrasonography evaluation. RESULTS: The 3-microm microparticles (3.26-microm mean diameter with 0.41-microm standard deviation) led to in vitro enhancement of 18.3 dB at 0.62 mg/mL. In vivo experiments showed 47% enhancement of intratumoral vascularization detection after PLGA injection, significantly correlated (P < 0.0001) with preinjection intravascularization and tumoral volume. No toxicity was histologically observed. CONCLUSION: The 3-microm PLGA microparticles provided significant enhancement in vitro and in vivo without any toxicity. |
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