| 米力农吸入对急性肺损伤家兔肺iNOS和eNOS表达的影响 |
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| 引用本文： | 刘霖,王智超,李立为,袁帅.米力农吸入对急性肺损伤家兔肺iNOS和eNOS表达的影响[J].临床急诊杂志,2011,12(3):166-168. |
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| 作者姓名： | 刘霖 王智超 李立为 袁帅 |
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| 作者单位： | 华中科技大学附属中西医结合医院急诊科,武汉,430023 |
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| 摘 要： | 目的:观察米力农吸入对急性肺损伤(ALI)家兔肺iNOS和eNOS表达的影响并探讨其保护机制。方法:将45只家兔随机分为对照组(A组),肺损伤组(B组)和米力农吸入组(C组),每组15只,B和C组采用特制多功能撞击机制成急性肺损伤模型,C组经气管给予米力农雾化吸入,分别在0、1、2、3、4h时间点行血气分析,4h后处死动物,测定肺湿干比(W/D)、血清NO、肺组织丙二醛(MDA)和髓过氧化物酶(MPO)含量,免疫组化法检测肺组织iNOS和eNOS表达。结果:与A组相比,B组损伤后PaO2、SaO2显著降低(P<0.05),肺iNOS表达显著增强,eNOS表达明显降低(P<0.05),肺W/D、血清NO、肺MDA和MPO含量显著增高(P<0.05)。C组米力农吸入后,与B组比较,PaO2、SaO2明显升高(P<0.05),肺iNOS表达显著降低,eNOS表达明显升高(P<0.05),肺W/D、血清NO、肺MDA和MPO含量明显降低(P<0.05)。结论:米力农通过抑制肺iNOS异常高表达和增加eNOS表达对急性肺损伤有保护作用。
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| 关 键 词： | 米力农 急性肺损伤 一氧化氮合酶 |
Effect of milrinone inhalation on expression of lung tissue iNOS and eNOS during acute lung injury in rabbits |
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| LIU Lin,WANG Zhichao,LI Liwei,YUAN Shuai.Effect of milrinone inhalation on expression of lung tissue iNOS and eNOS during acute lung injury in rabbits[J].Journal of Clinical Emergency Call,2011,12(3):166-168. |
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| Authors: | LIU Lin WANG Zhichao LI Liwei YUAN Shuai |
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| Institution: | LIU Lin WANG Zhichao LI Liwei YUAN Shuai(Department of Emergency,the Hospital of Traditional Chinese and Western Medicine,Huazhong University of Science and Technology,Wuhan,430023,China) |
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| Abstract: | Objective:To investigate the effect of milrinone inhalation on expression of lung tissue iNOS and eNOS during acute lung injury in rabbits and explore it's protective mechanism.Method:Forty-five rabbits were randomly divided into three groups:control group(group A),ALI group(group B) and milrinone inhalation group(group C) with 15 in each group.ALI model was established by special equipment in group B and group C,and milrinone was applied in group C.Blood samples were taken for blood-gas analysis at 0,1,2,3... |
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| Keywords: | milrinone acute lung injury nitric oxide synthase |
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